Literature summary for 2.1.2.14 extracted from

Riegert, A.S.; Chantigian, D.P.; Thoden, J.B.; Tipton, P.A.; Holden, H.M.
Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis (2017), Biochemistry, 56, 3657-3668 .

Search for more literature for 2.1.2.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Brucella melitensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme D78A/F142A, hanging drop vapor diffusion method, using 13-15% (w/v) poly(ethylene glycol) 5000, 2% (v/v) glycerol. Wild type enzyme, hanging drop vapor diffusion method, using 12-15% (w/v) poly(ethylene glycol) 8000m and 2% (w/v) 2-methyl-2,4-pentanediol	Brucella melitensis

Protein Variants

Protein Variants	Comment	Organism
D78A/F142A	the mutant variant does not exhibit substrate inhibition, but does display kinetic behavior expected for an enzyme with modest cooperativity	Brucella melitensis

Inhibitors

Inhibitors	Comment	Organism	Structure
GDP-alpha-D-perosamine	substrate inhibition by GDP-alpha-D-perosamine is evident above 0.25 mM	Brucella melitensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.46	-	GDP-alpha-D-perosamine	mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication	Brucella melitensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
10-formyltetrahydrofolate + GDP-alpha-D-perosamine	Brucella melitensis	-	tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Brucella melitensis	Q9ZHX0	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Brucella melitensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
10-formyltetrahydrofolate + GDP-3-deoxyperosamine	-	Brucella melitensis	tetrahydrofolate + GDP-N-formyl-3-deoxyperosamine	-	?
10-formyltetrahydrofolate + GDP-alpha-D-perosamine	-	Brucella melitensis	tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine	-	?

Synonyms

Synonyms	Comment	Organism
wbkC	-	Brucella melitensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.54	-	GDP-alpha-D-perosamine	mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication	Brucella melitensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.2	-	GDP-alpha-D-perosamine	mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication	Brucella melitensis

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Release 2023.1 (January 2023)