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Literature summary for 2.1.2.10 extracted from

  • Okamura-Ikeda, K.; Fujiwara, K.; Motokawa, Y.
    Purification and characterization of chicken liver T-protein, a component of the glycine cleavage system (1982), J. Biol. Chem., 257, 135-139.
    View publication on PubMed

General Stability

General Stability Organism
lability of enzyme under the conditions of its purifications and storage Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
S-aminomethyldihydrolipoylprotein
-
Gallus gallus
0.17
-
tetrahydrofolate
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000 38000 sedimentation equilibrium centrifugation, gel filtration Gallus gallus
41000
-
1 * 41000, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate Gallus gallus part of the glycine cleavage system dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
641fold purification Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl buffer, pH 8.0, 10 mM 2-mercaptoethanol, 50% v/v glycerol, at least 2 months, stable Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information component of the glycine cleavage system, T-protein is associated with H-protein forming a complex which is composed of one molecule of each of them Gallus gallus ?
-
?
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates Gallus gallus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
r
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate strictly dependent on tetrahydrofolate Gallus gallus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
r
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate part of the glycine cleavage system Gallus gallus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 9.8 Gallus gallus

Cofactor

Cofactor Comment Organism Structure
tetrahydrofolate strictly dependent on tetrahydrofolate Gallus gallus