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Literature summary for 2.1.2.1 extracted from

  • Sodolescu, A.; Dian, C.; Terradot, L.; Bouzhir-Sima, L.; Lestini, R.; Myllykallio, H.; Skouloubris, S.; Liebl, U.
    Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori (2018), PLoS ONE, 13, e0208850 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli SG 13009 cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M sodium acetate Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Helicobacter pylori
-
 tetrahydrofolate + L-serine
-
 r
5,10-methylenetetrahydrofolate + glycine + H2O Helicobacter pylori 26695
-
 tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine Helicobacter pylori
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
tetrahydrofolate + L-serine Helicobacter pylori 26695
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Helicobacter pylori P56089
-
-
Helicobacter pylori 26695 P56089
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-TED column chromatography Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Helicobacter pylori tetrahydrofolate + L-serine
-
 r
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Helicobacter pylori 26695 tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine
-
 Helicobacter pylori 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
tetrahydrofolate + L-serine
-
 Helicobacter pylori 26695 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Helicobacter pylori
homodimer 2 * 46776, calculated from amino acid sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
GlyA
-
 Helicobacter pylori
serine hydroxymethyltransferase
-
 Helicobacter pylori
SHMT
-
 Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Helicobacter pylori