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Literature summary for 2.1.2.1 extracted from

  • Ruszkowski, M.; Sekula, B.; Ruszkowska, A.; Dauter, Z.
    Chloroplastic serine hydroxymethyltransferase from Medicago truncatula A structural characterization (2018), Front. Plant Sci., 9, 584 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 gold cells Medicago truncatula

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme,hanging drop vapor diffusion method, using 55% tacsimate pH 7.0. Enzyme bound to reaction intermediates, hanging drop vapor diffusion method, using 75 mM MES pH 6.5, 19%(w/v) polyethylene glycol 3350 and 150 mMammonium acetate Medicago truncatula

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Medicago truncatula 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Medicago truncatula
197000
-
theoretical molecular mass of the tetramer Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Medicago truncatula
-
tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine Medicago truncatula
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

Organism UniProt Comment Textmining
Medicago truncatula G7ILW0
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap resin column chromatography and Superdex S200 gel filtration Medicago truncatula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Medicago truncatula tetrahydrofolate + L-serine
-
r
tetrahydrofolate + L-serine
-
Medicago truncatula 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Subunits

Subunits Comment Organism
tetramer
-
Medicago truncatula

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Medicago truncatula
SHMT3
-
Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Medicago truncatula