Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 gold cells | Medicago truncatula |
Crystallization (Comment) | Organism |
---|---|
apoenzyme,hanging drop vapor diffusion method, using 55% tacsimate pH 7.0. Enzyme bound to reaction intermediates, hanging drop vapor diffusion method, using 75 mM MES pH 6.5, 19%(w/v) polyethylene glycol 3350 and 150 mMammonium acetate | Medicago truncatula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Medicago truncatula | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Medicago truncatula |
197000 | - |
theoretical molecular mass of the tetramer | Medicago truncatula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Medicago truncatula | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Medicago truncatula | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | G7ILW0 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap resin column chromatography and Superdex S200 gel filtration | Medicago truncatula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Medicago truncatula | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Medicago truncatula | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Medicago truncatula |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Medicago truncatula |
SHMT3 | - |
Medicago truncatula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Medicago truncatula |