Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-TIR cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 28% (w/v) PEGMME 2000 (in complex with lometrexol and methotrexate), 0.2 M LiCl2, 0.1 M MES pH 6.0, 20% (w/v) PEG6000 (in complex with pemetrexed), and 0.2 M NaCl, 0.1 M MES pH 6.0, 20% (w/v) PEG6000 (in complex with raltitrexed) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lometrexol | 49.7% residual activity at 0.1 mM | Homo sapiens | |
methotrexate | 87.4% residual activity at 0.1 mM | Homo sapiens | |
pemetrexed | 79.2% residual activity at 0.1 mM | Homo sapiens | |
raltitrexed | 94% residual activity at 0.1 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Homo sapiens | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Homo sapiens | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34897 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography and Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Homo sapiens | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
SHMT2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |