Literature summary for 2.1.2.1 extracted from

Amornwatcharapong, W.; Maenpuen, S.; Chitnumsub, P.; Leartsakulpanich, U.; Chaiyen, P.
Human and Plasmodium serine hydroxymethyltransferases differ in rate-limiting steps and pH-dependent substrate inhibition behavior (2017), Arch. Biochem. Biophys., 630, 91-100 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens
expressed in Escherichia coli BL21(DE3) cells	Plasmodium vivax

Inhibitors

Inhibitors	Comment	Organism	Structure
tetrahydrofolate	strong and pH-dependent substrate inhibition due to the formation of the enzyme-glycine:tetrahydrofolate dead-end complex (at low pH values)	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.005	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Homo sapiens
0.011	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Homo sapiens
0.018	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Homo sapiens
0.038	-	tetrahydrofolate	at pH 8.3, temperature not specified in the publication	Plasmodium vivax
0.042	-	tetrahydrofolate	at pH 7.9, temperature not specified in the publication	Plasmodium vivax
0.048	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Plasmodium vivax
0.049	-	tetrahydrofolate	at pH 7.9, temperature not specified in the publication	Homo sapiens
0.051	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Plasmodium vivax
0.054	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Plasmodium vivax
0.094	-	tetrahydrofolate	at pH 8.3, temperature not specified in the publication	Homo sapiens
0.11	-	L-serine	at pH 7.9, temperature not specified in the publication	Plasmodium vivax
0.12	-	L-serine	at pH 8.3, temperature not specified in the publication	Plasmodium vivax
0.16	-	L-serine	at pH 8.3, temperature not specified in the publication	Homo sapiens
0.18	-	L-serine	at pH 7.9, temperature not specified in the publication	Homo sapiens
0.2	-	L-serine	at pH 7.5, temperature not specified in the publication	Plasmodium vivax
0.22	-	L-serine	at pH 7.5, temperature not specified in the publication	Homo sapiens
0.23	-	L-serine	at pH 7.1, temperature not specified in the publication	Plasmodium vivax
0.28	-	L-serine	at pH 7.1, temperature not specified in the publication	Homo sapiens
0.3	-	L-serine	at pH 6.6, temperature not specified in the publication	Plasmodium vivax
0.42	-	L-serine	at pH 6.6, temperature not specified in the publication	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
5,10-methylenetetrahydrofolate + glycine + H2O	Homo sapiens	-	tetrahydrofolate + L-serine	-	r
5,10-methylenetetrahydrofolate + glycine + H2O	Plasmodium vivax	-	tetrahydrofolate + L-serine	-	r
tetrahydrofolate + L-serine	Homo sapiens	-	5,10-methylenetetrahydrofolate + glycine + H2O	-	r
tetrahydrofolate + L-serine	Plasmodium vivax	-	5,10-methylenetetrahydrofolate + glycine + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Plasmodium vivax	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
polyethyleneimine precipitation, Q-Sepharose column chromatography and Sephacyl S-200 gel filtration	Homo sapiens
polyethyleneimine precipitation, SP-Sepharose column chromatography and DEAE-Sepharose column chromatography	Plasmodium vivax

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
5,10-methylenetetrahydrofolate + glycine + H2O	-	Homo sapiens	tetrahydrofolate + L-serine	-	r
5,10-methylenetetrahydrofolate + glycine + H2O	-	Plasmodium vivax	tetrahydrofolate + L-serine	-	r
tetrahydrofolate + L-serine	-	Homo sapiens	5,10-methylenetetrahydrofolate + glycine + H2O	-	r
tetrahydrofolate + L-serine	-	Plasmodium vivax	5,10-methylenetetrahydrofolate + glycine + H2O	-	r

Synonyms

Synonyms	Comment	Organism
serine hydroxymethyltransferase	-	Homo sapiens
serine hydroxymethyltransferase	-	Plasmodium vivax
SHMT	-	Homo sapiens
SHMT	-	Plasmodium vivax

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.41	-	L-serine	at pH 8.3, temperature not specified in the publication	Plasmodium vivax
0.41	-	tetrahydrofolate	at pH 8.3, temperature not specified in the publication	Plasmodium vivax
0.57	-	L-serine	at pH 7.9, temperature not specified in the publication	Plasmodium vivax
0.57	-	tetrahydrofolate	at pH 7.9, temperature not specified in the publication	Plasmodium vivax
0.81	-	L-serine	at pH 7.5, temperature not specified in the publication	Plasmodium vivax
0.81	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Plasmodium vivax
1.1	-	L-serine	at pH 6.6, temperature not specified in the publication	Plasmodium vivax
1.1	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Plasmodium vivax
1.41	-	L-serine	at pH 7.1, temperature not specified in the publication	Plasmodium vivax
1.41	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Plasmodium vivax
19.6	-	L-serine	at pH 8.3, temperature not specified in the publication	Homo sapiens
19.6	-	tetrahydrofolate	at pH 8.3, temperature not specified in the publication	Homo sapiens
19.8	-	L-serine	at pH 7.5, temperature not specified in the publication	Homo sapiens
19.8	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Homo sapiens
19.8	-	L-serine	at pH 7.9, temperature not specified in the publication	Homo sapiens
19.8	-	tetrahydrofolate	at pH 7.9, temperature not specified in the publication	Homo sapiens
21	-	L-serine	at pH 7.1, temperature not specified in the publication	Homo sapiens
21	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Homo sapiens
30	-	L-serine	at pH 6.6, temperature not specified in the publication	Homo sapiens
30	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	-	Homo sapiens
7	-	-	Plasmodium vivax

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.6	8.4	the catalytic efficiency of the enzyme for tetrahydrofolate is about 29fold greater at pH 6.6 than at pH 8.3	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Homo sapiens
pyridoxal 5'-phosphate	-	Plasmodium vivax

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.14	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Homo sapiens
0.16	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Homo sapiens
0.27	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
3.4	-	L-serine	at pH 8.3, temperature not specified in the publication	Plasmodium vivax
3.7	-	L-serine	at pH 6.6, temperature not specified in the publication	Plasmodium vivax
4.1	-	L-serine	at pH 7.5, temperature not specified in the publication	Plasmodium vivax
5.2	-	L-serine	at pH 7.9, temperature not specified in the publication	Plasmodium vivax
6.1	-	L-serine	at pH 7.1, temperature not specified in the publication	Plasmodium vivax
11	-	tetrahydrofolate	at pH 8.3, temperature not specified in the publication	Plasmodium vivax
14	-	tetrahydrofolate	at pH 7.9, temperature not specified in the publication	Plasmodium vivax
15	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Plasmodium vivax
22	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Plasmodium vivax
29	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Plasmodium vivax
71	-	L-serine	at pH 6.6, temperature not specified in the publication	Homo sapiens
75	-	L-serine	at pH 7.1, temperature not specified in the publication	Homo sapiens
90	-	L-serine	at pH 7.5, temperature not specified in the publication	Homo sapiens
110	-	L-serine	at pH 7.9, temperature not specified in the publication	Homo sapiens
123	-	L-serine	at pH 8.3, temperature not specified in the publication	Homo sapiens
209	-	tetrahydrofolate	at pH 8.3, temperature not specified in the publication	Homo sapiens
404	-	tetrahydrofolate	at pH 7.9, temperature not specified in the publication	Homo sapiens
1100	-	tetrahydrofolate	at pH 7.5, temperature not specified in the publication	Homo sapiens
1909	-	tetrahydrofolate	at pH 7.1, temperature not specified in the publication	Homo sapiens
6000	-	tetrahydrofolate	at pH 6.6, temperature not specified in the publication	Homo sapiens