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Literature summary for 2.1.2.1 extracted from

  • Chitnumsub, P.; Jaruwat, A.; Riangrungroj, P.; Ittarat, W.; Noytanom, K.; Oonanant, W.; Vanichthanankul, J.; Chuankhayan, P.; Maenpuen, S.; Chen, C.J.; Chaiyen, P.; Yuthavong, Y.; Leartsakulpanich, U.
    Structures of Plasmodium vivax serine hydroxymethyltransferase: implications for ligand-binding specificity and functional control (2014), Acta Crystallogr. Sect. D, 70, 3177-3186.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme in a binary complex with L-serine and in a ternary complex with D-serine and (6R)-5-formyltetrahydrofolate, microbatch method, mixing of 0.001 ml of 0.38 mM protein in 0.86 mM PLP, 62 mM 2-mercaptoethanol, 87 mM Gly, L-Ser, or D-Ser, and 43 mM tetrahydrofolate, with 0.001 ml of well solution containing 20-21% w/v PEG 4000, 70-90 mM NaCl, 100 mM Tris-HCl, pH 8.5, 15% v/v trifluoroethanol, 20°C, 1-4 days, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, molecular replacement Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Plasmodium vivax the enzyme catalyzes the conversion of L- and D-serine to glycine in a THFdependent reaction ?
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium vivax A5K8L9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium vivax tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium vivax tetrahydrofolate + D-serine
-
r
additional information the enzyme catalyzes the conversion of L- and D-serine to glycine in a THFdependent reaction Plasmodium vivax ?
-
?
additional information L- and D-serine substrate binding structure analysis, overview Plasmodium vivax ?
-
?

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Plasmodium vivax
SHMT
-
Plasmodium vivax

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Plasmodium vivax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Plasmodium vivax

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Plasmodium vivax
pyridoxal 5'-phosphate
-
Plasmodium vivax
tetrahydrofolate binding structure analysis Plasmodium vivax

General Information

General Information Comment Organism
metabolism the enzyme is involved in folate recycling and dTMP synthesis Plasmodium vivax