Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant dominant negative SHMT1, DN2-SHMT1, localizes with lamin B1 and enhances SHMT1 activity for de novo thymidylate biosynthesis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of a dominant negative, tetracycline-inducible SHMT1 protein, DN2-SHMT1, in HeLa and SH-SY5Y cells, expression of RFP-tagged SHMT2alpha and of YFP- or FLAG-tagged SHMT1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | SHMT1 encodes the cytoplasmic/nuclear isozyme SHMT1, SHMT2 encodes the mitochondrial isozyme SHMT2 and the cytoplasmic/nuclear isozyme SHMT2alpha through alternative promoter uses | Homo sapiens | 5737 | - |
mitochondrion | SHMT2 encodes the mitochondrial isozyme SHMT2 and the cytoplasmic/nuclear isozyme SHMT2alpha through alternative promoter uses | Homo sapiens | 5739 | - |
nucleus | SHMT1 encodes the cytoplasmic/nuclear isozyme SHMT1, SHMT2 encodes the mitochondrial isozyme SHMT2 and the cytoplasmic/nuclear isozyme SHMT2alpha through alternative promoter uses. SHMT1, SHMT2alpha, thymidylate synthase, and dihydrofolate reductase are present in nuclei during S and G2/M phases | Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes by tandem affinity purification, immunoprecipitation, heat treatment, | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | SHMT2alpha co-localizes with lamin B1 in SH-SY5Y cells. Recombinant dominant negative SHMT1, DN2-SHMT1, localizes with lamin B1 and enhances SHMT1 activity for de novo thymidylate biosynthesis | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
serine hydroxymethyltransferase 1 | - |
Homo sapiens |
serine hydroxymethyltransferase 2alpha | - |
Homo sapiens |
SHMT | - |
Homo sapiens |
SHMT1 | - |
Homo sapiens |
SHMT2alpha | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the de novo thymidylate biosynthetic pathway forms a multienzyme complex, containing enzymes serine hydroxymethyltransferase 1 and 2alpha, thymidylate synthase, and dihydrofolate reductase, the complex is associated with the nuclear lamina, overview. The de novo thymidylate biosynthetic pathway in mammalian cells translocates to the nucleus for DNA replication and repair. SHMT1 or SHMT2alpha are required for co-localization of dihydrofolate reductase, SHMT, and thymidylate synthase to the nuclear lamina, indicating that SHMT serves as scaffold protein that is essential for complex formation, SHMT1 scaffold function can determine de novo thymidylate synthesis capacity, SHMT1 interaction with TYMS and DHFR is DNA-dependent, but the formation of thymidylate biosynthesis complex is nucleotide-independent. Folate-mediated one-carbon metabolism in the cytoplasm and nucleus, overview | Homo sapiens |
physiological function | functional redundancy of SHMT2alpha and SHMT1 in nuclear de novo thymidylate synthesis. The de novo thymidylate biosynthetic pathway forms a multienzyme complex, containing enzymes serine hydroxymethyltransferase 1 and 2alpha, thymidylate synthase, and dihydrofolate reductase, the complex is associated with the nuclear lamina, overview. The de novo thymidylate biosynthetic pathway in mammalian cells translocates to the nucleus for DNA replication and repair. SHMT1 or SHMT2alpha are required for co-localization of dihydrofolate reductase, SHMT, and thymidylate synthase to the nuclear lamina, indicating that SHMT serves as scaffold protein that is essential for complex formation. SHMT expression is rate-limiting for de novo thymidylate synthesis | Homo sapiens |