Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and GS245(DE3) cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1843U89 | - |
Plasmodium falciparum | |
AG331 | - |
Plasmodium falciparum | |
AG337 | - |
Plasmodium falciparum | |
D1694 | - |
Plasmodium falciparum | |
GR1 | - |
Plasmodium falciparum | |
methotrexate | - |
Plasmodium falciparum | |
permetrexed | - |
Plasmodium falciparum | |
pyrimethamine | - |
Plasmodium falciparum | |
WR99210 | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-serine | in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | |
3.4 | - |
tetrahydrofolate | in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, His-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
81500 | - |
calculated molecular weight | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the His-tagged enzyme is purified by His GraviTrap column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Plasmodium falciparum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 55000, His-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Plasmodium falciparum |
SHMT | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
L-serine | in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, the apo enzyme has some enzyme activity in the absence of pyridoxal 5'-phosphate but this activity is only slightly above background and can be due to incomplete removal of pyridoxal 5'-phosphate | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.125 | - |
in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | WR99210 | |
0.15 | - |
in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | 1843U89 | |
0.15 | - |
in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | GR1 | |
0.15 | - |
in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | AG331 | |
0.2 | - |
in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | AG337 | |
0.5 | - |
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | methotrexate | |
0.5 | - |
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | pyrimethamine | |
0.5 | - |
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | permetrexed | |
0.5 | - |
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C | Plasmodium falciparum | D1694 |