Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.2.1 extracted from

  • Pang, C.K.; Hunter, J.H.; Gujjar, R.; Podutoori, R.; Bowman, J.; Mudeppa, D.G.; Rathod, P.K.
    Catalytic and ligand-binding characteristics of Plasmodium falciparum serine hydroxymethyltransferase (2009), Mol. Biochem. Parasitol., 168, 74-83.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) and GS245(DE3) cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
1843U89
-
Plasmodium falciparum
AG331
-
Plasmodium falciparum
AG337
-
Plasmodium falciparum
D1694
-
Plasmodium falciparum
GR1
-
Plasmodium falciparum
methotrexate
-
Plasmodium falciparum
permetrexed
-
Plasmodium falciparum
pyrimethamine
-
Plasmodium falciparum
WR99210
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
L-serine in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum
3.4
-
tetrahydrofolate in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, His-tagged enzyme, SDS-PAGE Plasmodium falciparum
81500
-
calculated molecular weight Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the His-tagged enzyme is purified by His GraviTrap column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
Plasmodium falciparum glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 55000, His-tagged enzyme, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Plasmodium falciparum
SHMT
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
L-serine in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on, the apo enzyme has some enzyme activity in the absence of pyridoxal 5'-phosphate but this activity is only slightly above background and can be due to incomplete removal of pyridoxal 5'-phosphate Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.125
-
in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum WR99210
0.15
-
in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum 1843U89
0.15
-
in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum GR1
0.15
-
in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum AG331
0.2
-
in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum AG337
0.5
-
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum methotrexate
0.5
-
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum pyrimethamine
0.5
-
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum permetrexed
0.5
-
IC50 above 0.5 mM, in 50 mM Tris-HCl pH 8.0, at 37°C Plasmodium falciparum D1694