Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leucovorin | leucovorin (N5-CHO-THF) exhibits a differential inhibition pattern: it significantly inhibits the aldol cleavage of serine catalyzed by zebrafish cytosolic SHMT but it inhibits to a lesser extent the reaction catalyzed by the mitochondrial isozyme. Approximately 70% and 30% inhibition are observed for zebrafish cytosolic- and zebrafish mitochondrial SHMT activities, respectively, in the presence of 70 mM leucovorin. An even larger difference between both isoenzymes is observed when the inhibition is assayed in the presence of 50 mM serine; leucovorin (N5-CHO-THF) exhibits a differential inhibition pattern: it significantly inhibits the aldol cleavage of serine catalyzed by zebrafish cytosolic SHMT but it inhibits to a lesser extent the reaction catalyzed by the mitochondrial isozyme. Approximately 70% and 30% inhibition are observed for zebrafish cytosolic- and zebrafish mitochondrial SHMT activities, respectively, in the presence of 70 mM leucovorin. An even larger difference between both isoenzymes is observed when the inhibition is assayed in the presence of 50 mM serine | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
serine | - |
Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | zcSHMT is detected in the cytosol as well as in the nucleus | Danio rerio | 5829 | - |
mitochondrion | peptide sequence alignment with the known SHMT from other species and the prospective zebrafish cytosolic SHMT as well as signal prediction software reveals a potential mitochondrial signal peptide cleavage site between residues 20 and 30 | Danio rerio | 5739 | - |
nucleus | zcSHMT is detected in the cytosol as well as in the nucleus | Danio rerio | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | A9LDD9 | - |
- |
Danio rerio | Q7SXN1 | - |
- |
Purification (Comment) | Organism |
---|---|
using a 30 to 50% ammonium sulfate precipitation, gel filtration and chromatography on CM-Sephadex columns. From 2 liters of cells, approximately 43 mg of pure zmSHMT is obtained with an overall yield of 90% | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | elevated mRNA expression detected by RT-PCT | Danio rerio | - |
liver | elevated mRNA expression detected by RT-PCT. Strong protein expression detected by western blotting | Danio rerio | - |
liver | siginificant protein expression detected by Western Blotting only in liver and ovary | Danio rerio | - |
additional information | RT-PCR results show that zmSHMT mRNA is evenly distributed among tissues. However zmSHMT protein expression does not correspond to the detected mRNA expression detected in most tissues | Danio rerio | - |
ovary | elevated mRNA expression detected by RT-PCT. Strong protein expression detected by western blotting | Danio rerio | - |
ovary | siginificant protein expression detected by Western Blotting only in liver and ovary | Danio rerio | - |
Storage Stability | Organism |
---|---|
-20°C (or -80°C), in the presence of 10% glycerol, for at least 6 months, no significant change in catalytic activity | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Danio rerio | tetrahydrofolate + L-serine | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, gel filtration | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
zcSHMT | - |
Danio rerio |
zebrafish cytosolic serine hydroxymethyltransferase | - |
Danio rerio |
zebrafish mitochondiral serine hydroxymethyltransferase | - |
Danio rerio |
zmSHMT | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.95 | - |
serine | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Danio rerio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Danio rerio |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
30 | - |
- |
Danio rerio | leucovorin | |
70 | - |
- |
Danio rerio | leucovorin |