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Literature summary for 2.1.2.1 extracted from

  • Chang, W.N.; Tsai, J.N.; Chen, B.H.; Fu, T.F.
    Cloning, expression, purification, and characterization of zebrafish cytosolic serine hydroxymethyltransferase (2006), Protein Expr. Purif., 46, 212-220.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information higher concentrations of IPTG, increased induction temperature and/or prolonged induction time increase the ratio of insoluble and soluble SHMT-1 Danio rerio

Application

Application Comment Organism
medicine animal model for drug screening of SHMT Danio rerio

Cloned(Commentary)

Cloned (Comment) Organism
into pET43.1a vector and transformed into Escherichia coli HMS174(DE3) cells Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
L-serine
-
Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Danio rerio 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE, x * 53317, sequence analysis Danio rerio
53317
-
x * 53000, SDS-PAGE, x * 53317, sequence analysis Danio rerio
200000
-
gel filtration, holo- and apoenzyme Danio rerio

Organism

Organism UniProt Comment Textmining
Danio rerio Q2TL58 AB strain
-
Danio rerio AB Q2TL58 AB strain
-

Purification (Commentary)

Purification (Comment) Organism
three-step purification, gel filtration, 14.8fold, more than 95% pure Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Danio rerio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.46
-
purified enzyme Danio rerio

Storage Stability

Storage Stability Organism
-20 or -80°C, 10% glycerol, for at least 6 months Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
Danio rerio glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
L-serine + tetrahydrofolate
-
Danio rerio AB glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Subunits

Subunits Comment Organism
tetramer x * 53000, SDS-PAGE, x * 53317, sequence analysis Danio rerio

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Danio rerio
SHMT-1
-
Danio rerio

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
28 37
-
Danio rerio

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.85
-
L-serine
-
Danio rerio