Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | higher concentrations of IPTG, increased induction temperature and/or prolonged induction time increase the ratio of insoluble and soluble SHMT-1 | Danio rerio |
Application | Comment | Organism |
---|---|---|
medicine | animal model for drug screening of SHMT | Danio rerio |
Cloned (Comment) | Organism |
---|---|
into pET43.1a vector and transformed into Escherichia coli HMS174(DE3) cells | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-serine | - |
Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Danio rerio | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE, x * 53317, sequence analysis | Danio rerio |
53317 | - |
x * 53000, SDS-PAGE, x * 53317, sequence analysis | Danio rerio |
200000 | - |
gel filtration, holo- and apoenzyme | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q2TL58 | AB strain | - |
Danio rerio AB | Q2TL58 | AB strain | - |
Purification (Comment) | Organism |
---|---|
three-step purification, gel filtration, 14.8fold, more than 95% pure | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Danio rerio | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.46 | - |
purified enzyme | Danio rerio |
Storage Stability | Organism |
---|---|
-20 or -80°C, 10% glycerol, for at least 6 months | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Danio rerio | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | - |
Danio rerio AB | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x * 53000, SDS-PAGE, x * 53317, sequence analysis | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Danio rerio |
SHMT-1 | - |
Danio rerio |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 37 | - |
Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.85 | - |
L-serine | - |
Danio rerio |