Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.2.1 extracted from

  • Eisenhut, M.; Kahlon, S.; Hasse, D.; Ewald, R.; Lieman-Hurwitz, J.; Ogawa, T.; Ruth, W.; Bauwe, H.; Kaplan, A.; Hagemann, M.
    The plant-like C2 glycolate cycle and the bacterial-like glycerate pathway cooperate in phosphoglycolate metabolism in cyanobacteria (2006), Plant Physiol., 142, 333-342.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation essential for one-carbon metabolism Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed as fusion protein with an N-terminal Strep-tag in Escherichia coli strain BL21 Gold Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information SHMT mutant with only partial segregation Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, Western blot analysis Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
Synechocystis sp. glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, Western blot analysis Synechocystis sp.

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Synechocystis sp.
SHMT
-
Synechocystis sp.