Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal phosphate | - |
Trichomonas vaginalis |
Application | Comment | Organism |
---|---|---|
medicine | enzyme is distinct from its prokaryotic and eukaryotic counterparts, thus is a potential drug target | Trichomonas vaginalis |
Cloned (Comment) | Organism |
---|---|
wild-type and mutants cloned into vector pET-29B and expressed in Escherichia coli BL21(DE3) cells, moreover expressed in Trichomonas vaginalis with a C-terminal HA tag | Trichomonas vaginalis |
Protein Variants | Comment | Organism |
---|---|---|
K251Q | oligomeric structure not affected, but inactive in both the presence and absence of pyridoxal phosphate | Trichomonas vaginalis |
K251R | oligomeric structure not affected, catalytic activity in the presence of pyridoxal phosphate also unaffected | Trichomonas vaginalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
tetrahydrofolate | mutant K251R, without pyridoxal phosphate | Trichomonas vaginalis | |
0.89 | - |
tetrahydrofolate | wild-type, in the presence of 0.25 mM pyridoxal phosphate | Trichomonas vaginalis | |
1 | - |
tetrahydrofolate | wild-type, without pyridoxal phosphate | Trichomonas vaginalis | |
1.16 | - |
tetrahydrofolate | mutant K251R, in the presence of 0.25 mM pyridoxal phosphate | Trichomonas vaginalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
hydrogenosome | enzyme possesses a putative N-terminal hydrogenosomal presequence | Trichomonas vaginalis | 42566 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
sequence analysis | Trichomonas vaginalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichomonas vaginalis | - |
strains T1 and G3 | - |
Purification (Comment) | Organism |
---|---|
by Ni affinity chromatography, more than 98% pure | Trichomonas vaginalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Trichomonas vaginalis | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | immunoblotting | Trichomonas vaginalis |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyl transferase | - |
Trichomonas vaginalis |
SHMT | - |
Trichomonas vaginalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
tetrahydrofolate | mutant K251R, without pyridoxal phosphate | Trichomonas vaginalis | |
1.13 | - |
tetrahydrofolate | mutant K251R, in the presence of 0.25 mM pyridoxal phosphate | Trichomonas vaginalis | |
1.29 | - |
tetrahydrofolate | wild-type, without pyridoxal phosphate | Trichomonas vaginalis | |
1.3 | - |
tetrahydrofolate | wild-type, in the presence of 0.25 mM pyridoxal phosphate | Trichomonas vaginalis |