Any feedback?
Literature summary for 2.1.2.1 extracted from
- Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii (2003), J. Biol. Chem., 278, 41789-41797.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanocaldococcus jannaschii |
General Stability
| General Stability | Organism |
|---|---|
| above 6 M urea the dichrpoic activity is reduced significantly with a transition midpoint at 2.56 M. This process is completely reversible | Methanocaldococcus jannaschii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
tetrahydromethanopterin | pH 7.4, 37°C | Methanocaldococcus jannaschii |  |
| 0.8 | - |
L-Ser | pH 7.4, 37°C | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48200 | - |
2 * 48200, SDS-PAGE | Methanocaldococcus jannaschii |
| 98000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrahydromethanopterin + L-Ser | - |
Methanocaldococcus jannaschii | glycine + ? | - |
? | |
| tetrahydropteroylglutamate + L-Ser | - |
Methanocaldococcus jannaschii | glycine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48200, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
retroaldol cleavage of L-allo-threonine | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.53 | - |
L-Ser | pH 7.4, 37°C | Methanocaldococcus jannaschii | |
| 3.53 | - |
tetrahydromethanopterin | pH 7.4, 37°C | Methanocaldococcus jannaschii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
