Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-serine | only tetrameric form | Geobacillus stearothermophilus | |
0.9 | - |
L-serine | only dimeric form | Geobacillus stearothermophilus | |
1 | - |
L-serine | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Geobacillus stearothermophilus |
90000 | - |
dimeric form | Geobacillus stearothermophilus |
180000 | - |
tetrameric form, gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Geobacillus stearothermophilus | - |
glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gene cloned and expressed in Escherichia coli | Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine | mechanism | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
only tetrameric form | Geobacillus stearothermophilus |
4.2 | - |
- |
Geobacillus stearothermophilus |
6.7 | - |
only dimeric form | Geobacillus stearothermophilus |
32.4 | - |
dimeric form at 80°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Geobacillus stearothermophilus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | enzyme catalyses the racemization of D- and L-alanine | Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 90% dimer and 10% tetramer in the same organism, one subunit: 45000 Da, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
dimeric form of enzyme | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Tm values of enzyme and dimeric and tetrameric forms in the absence and presence of ligands | Geobacillus stearothermophilus |
additional information | - |
pyridoxal 5'-phosphate increases the thermal stability | Geobacillus stearothermophilus |
additional information | - |
L-serine increases the thermal stability | Geobacillus stearothermophilus |
60 | - |
enzyme stable in the absence of any ligand | Geobacillus stearothermophilus |
70 | - |
50% loss of activity in the absence of any ligand | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
L-serine | only tetrameric form | Geobacillus stearothermophilus | |
4.3 | - |
L-serine | - |
Geobacillus stearothermophilus | |
5 | - |
L-serine | only dimeric form | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Geobacillus stearothermophilus |