Cloned (Comment) | Organism |
---|---|
Escherichia coli glyA gene | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
D-alanine | inactivates enzyme by converting the enzyme bound pyridoxal 5'-phosphate to pyridoxamine phosphate in a transamination reaction | Escherichia coli | |
methyl methanethiosulfonate | - |
Escherichia coli | |
additional information | not: iodoacetate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.08 | - |
tetrahydrofolate | - |
Escherichia coli | |
0.15 | 0.9 | L-serine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, SDS-PAGE | Escherichia coli |
47000 | - |
2 * 47000, calculated from DNA-sequence | Escherichia coli |
96000 | - |
sedimentation equilibrium centrifugation, amino acid composition | Escherichia coli |
100000 | 115000 | HPLC gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Escherichia coli | major pathway for production of C1-units of 5,10-methylenetetrahydrofolate | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine | mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.6 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Escherichia coli | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
L-serine + tetrahydrofolate | major pathway for production of C1-units of 5,10-methylenetetrahydrofolate | Escherichia coli | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, SDS-PAGE | Escherichia coli |
dimer | 2 * 47000, calculated from DNA-sequence | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
10.7 | - |
L-serine | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | stable | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli | |
tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Escherichia coli | |
tetrahydrofolate derivatives | requirement, if L-serine is substrate | Escherichia coli |