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Literature summary for 2.1.2.1 extracted from
- Serine hydroxymethyltransferase from Escherichia coli: purification and properties (1985), J. Bacteriol., 163, 1-7.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli glyA gene | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli |  |
| D-alanine | inactivates enzyme by converting the enzyme bound pyridoxal 5'-phosphate to pyridoxamine phosphate in a transamination reaction | Escherichia coli | |
| methyl methanethiosulfonate | - |
Escherichia coli | |
| additional information | not: iodoacetate | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| 0.08 | - |
tetrahydrofolate | - |
Escherichia coli | |
| 0.15 | 0.9 | L-serine | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, SDS-PAGE | Escherichia coli |
| 47000 | - |
2 * 47000, calculated from DNA-sequence | Escherichia coli |
| 96000 | - |
sedimentation equilibrium centrifugation, amino acid composition | Escherichia coli |
| 100000 | 115000 | HPLC gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | Escherichia coli | major pathway for production of C1-units of 5,10-methylenetetrahydrofolate | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine | mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.6 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | - |
Escherichia coli | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
| L-serine + tetrahydrofolate | major pathway for production of C1-units of 5,10-methylenetetrahydrofolate | Escherichia coli | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
| additional information | enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, SDS-PAGE | Escherichia coli |
| dimer | 2 * 47000, calculated from DNA-sequence | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| 10.7 | - |
L-serine | - |
Escherichia coli | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | stable | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
| tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Escherichia coli | |
| tetrahydrofolate derivatives | requirement, if L-serine is substrate | Escherichia coli |
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