Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-Alanine | weak | Rattus norvegicus | |
beta-Aminoalanine | - |
Rattus norvegicus | |
glycine | - |
Rattus norvegicus | |
L-alanine | - |
Rattus norvegicus | |
L-amino acids | weak, e.g. L-aspartic acid, ornithine, lysine, methionine, phenylalanine, homoserine, threonine, 4-aminobutyric acid | Rattus norvegicus | |
L-cysteine | mitochondrial enzyme | Rattus norvegicus | |
L-serine | strong | Rattus norvegicus | |
additional information | not: mercaptopropionic acid, mercaptoethanolamine; not: valine, leucine, glutamic acid, 3-hydroxybutyric acid | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | 0.9 | L-serine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
additional information | comparison of cytosolic and mitochondrial forms of enzyme | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
4 * 54000, SDS-PAGE | Rattus norvegicus |
220000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Rattus norvegicus | major pathway for production of C1-units of 5,10-methylenetetrahydrofolate | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on L-adsorbent Sepharose | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Rattus norvegicus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
L-serine + tetrahydrofolate | major pathway for production of C1-units of 5,10-methylenetetrahydrofolate | Rattus norvegicus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | no: L-threonine | Rattus norvegicus | ? | - |
? | |
additional information | no: D-serine | Rattus norvegicus | ? | - |
? | |
additional information | no: D-allothreonine | Rattus norvegicus | ? | - |
? | |
additional information | no: D-threonine | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 54000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI, cytosolic enzyme: 4.5, pI, mitochondrial enzyme: 4.8 | Rattus norvegicus |
6.8 | 8 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, 4 mol per mol enzyme | Rattus norvegicus | |
tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Rattus norvegicus |