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Literature summary for 2.1.1.B128 extracted from

  • Sharma, S.; Hartmann, J.; Watzinger, P.; Klepper, A.; Peifer, C.; Koetter, P.; Lafontaine, D.; Entian, K.
    A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes (2018), Sci. Rep., 8, 11904 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B128

Cloned(Commentary)

Cloned (Comment) Organism
gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis, recombinant expression in Saccharomyces cereviaie Rrp8 deficient mutant strain without complementation by the human Rrp8 enzyme Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information siRNA-mediated depletion of NML in HCT-116 cells, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleolus NML is primarily localized in the nucleolus Homo sapiens 5730
-
nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287 ?
-
-
S-adenosyl-L-methionine + adenine1322 in 28S rRNA Homo sapiens
-
 S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43159
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HCT-116 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287 Homo sapiens ?
-
-
S-adenosyl-L-methionine + adenine1322 in 28S rRNA
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
m1A large subunit rRNA methyltransferase
-
 Homo sapiens
More cf. EC 2.1.1.287 Homo sapiens
NML
-
 Homo sapiens
nucleomethylin
-
 Homo sapiens
ribosomal RNA-processing protein 8 UniProt Homo sapiens
Rrp8
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
evolution the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved Homo sapiens
malfunction siRNA-mediated depletion of NML is highly efficient at mRNA level, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells. Depletion of NML leads to abnormal and altered nucleolar morphology Homo sapiens
physiological function the human nucleomethylin (NML or RRP8) fails to complement the pre-rRNA processing phenotype and the growth cold sensitivity of Saccharomyces cerevisiae DELTArrp8 mutant strain Homo sapiens