Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 50% inhibition observed at 0.3 mM | Pyrococcus abyssi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000159 | - |
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | pH 7.5, 75°C | Pyrococcus abyssi | |
0.00128 | - |
S-adenosyl-L-methionine | pH 7.5, 75°C | Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by Mg2+, Ca2+, or Mn2+ | Pyrococcus abyssi | |
NaCl | activity is optimal at an NaCl concentration of 200 to 500 mM | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Pyrococcus abyssi |
52455 | - |
1 * 52455, calculated from sequence | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | - |
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9V2B5 | - |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | Q9V2B5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
? | |
S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52455, calculated from sequence | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
5'-GTAC-3'N6-adenine methyltransferase | - |
Pyrococcus abyssi |
M.PabI | - |
Pyrococcus abyssi |
M.PabI | PabI constitutes a type II restriction-modification gene complex | Pyrococcus abyssi |
PAB2246 | locus name | Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Pyrococcus abyssi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 95 | 65°C: about 40% of maximal activity, 95°C: about 40% of maximal activity | Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
9 min, 50% of the activity is retained | Pyrococcus abyssi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | pH 7.5, 75°C | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.7 | at 75°C | Pyrococcus abyssi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | pH 3.0: about 55% of maximal activity, pH 7.5: about 50% of maximal activity | Pyrococcus abyssi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
257 | - |
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | pH 7.5, 75°C | Pyrococcus abyssi |