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Literature summary for 2.1.1.B112 extracted from

  • Niu, Y.; Xia, Y.; Wang, S.; Li, J.; Niu, C.; Li, X.; Zhao, Y.; Xiong, H.; Li, Z.; Lou, H.; Cao, Q.
    A prototypic lysine methyltransferase 4 from archaea with degenerate sequence specificity methylates chromatin proteins Sul7d and Cren7 in different patterns (2013), J. Biol. Chem., 288, 13728-13740.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
DNA increased activity on Sul7d, but not Cren7, in the presence of DNA Sulfolobus islandicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus islandicus

Protein Variants

Protein Variants Comment Organism
G38A methyltransferase activity is completely abolished Sulfolobus islandicus
additional information aKMT4-8A mutant (a mutant with all eight lysine residues mutated to alanine): automethylation is almost completely removed in the presence of substrates, such as Cren7 and Sul7d. Retains significant automethylation activity in the absence of substrates. Mutant with abrogated automethylation shows a more than 150% increase in methylation of substrates Sulfolobus islandicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00112
-
[Sul7d-chromatin-protein] L-lysine pH 8.0, 50°C, in presence of DNA Sulfolobus islandicus
0.00397
-
[Sul7d-chromatin-protein] L-lysine pH 8.0, 50°C, in absence of DNA Sulfolobus islandicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine Sulfolobus islandicus the enzyme methylates Sul7d in the chromatin context S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine Sulfolobus islandicus Rey15A the enzyme methylates Sul7d in the chromatin context S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Sulfolobus islandicus F0NBH8
-
-
Sulfolobus islandicus Rey15A F0NBH8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus islandicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme can self-methylate intramolecularly. The substrates Sul7d, RPL11, and Cren7 inhibit automethylation in a concentration-dependent manner. This suggests that the enzyme transfers a methyl group from incoming S-adenosyl-L-methionine to itself in the absence of substrates. When the enzyme associates with the proper substrates, however, the enzyme prefers to transfer the methyl group to the substrates rather than to itself Sulfolobus islandicus ?
-
?
additional information the enzyme can self-methylate intramolecularly. The substrates Sul7d, RPL11, and Cren7 inhibit automethylation in a concentration-dependent manner. This suggests that the enzyme transfers a methyl group from incoming S-adenosyl-L-methionine to itself in the absence of substrates. When the enzyme associates with the proper substrates, however, the enzyme prefers to transfer the methyl group to the substrates rather than to itself Sulfolobus islandicus Rey15A ?
-
?
S-adenosyl-L-methionine + [Cren7-chromatin-protein] L-lysine
-
Sulfolobus islandicus S-adenosyl-L-homocysteine + [Cren7-chromatin-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Cren7-chromatin-protein] L-lysine
-
Sulfolobus islandicus Rey15A S-adenosyl-L-homocysteine + [Cren7-chromatin-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Csl4-protein] L-lysine
-
Sulfolobus islandicus S-adenosyl-L-homocysteine + [Csl4-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Csl4-protein] L-lysine
-
Sulfolobus islandicus Rey15A S-adenosyl-L-homocysteine + [Csl4-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Rrp4-protein] L-lysine
-
Sulfolobus islandicus S-adenosyl-L-homocysteine + [Rrp4-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Rrp42-protein] L-lysine
-
Sulfolobus islandicus S-adenosyl-L-homocysteine + [Rrp42-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine
-
Sulfolobus islandicus S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine the enzyme methylates Sul7d in the chromatin context Sulfolobus islandicus S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine
-
Sulfolobus islandicus Rey15A S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine the enzyme methylates Sul7d in the chromatin context Sulfolobus islandicus Rey15A S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine
-
?

Synonyms

Synonyms Comment Organism
aKMT4
-
Sulfolobus islandicus
SiRe_1449
-
Sulfolobus islandicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
Sulfolobus islandicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 40% of maximal activity, 80°C: about 40% of maximal activity Sulfolobus islandicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Sulfolobus islandicus