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Literature summary for 2.1.1.99 extracted from

  • Dethier, M.; De Luca, V.
    Partial purification of an N-methyltransferase involved in vindoline biosynthesis in Catharanthus roseus (1993), Phytochemistry, 32, 673-678.
No PubMed abstract available
Search for more literature for 2.1.1.99

Activating Compound

Activating Compound Comment Organism Structure
CHAPS highest activity with 3 mg/ml Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
lysolecithin completely elimination of activity Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast associated with thylakoids Catharanthus roseus 9507
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel chromatography Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine Catharanthus roseus enzyme is involved in the biosynthesis of vindoline from tabersonine S-adenosyl-L-homocysteine + deacetoxyvindoline
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 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information octyl-beta-D-glucoside: 0.15% solution 100% of activity Catharanthus roseus
additional information CHAPS: 232% activity is solubilized Catharanthus roseus
Triton X-100 0.1% Triton X-100 90% of activity Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
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-
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Purification (Commentary)

Purification (Comment) Organism
partially Catharanthus roseus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 3-hydroxy-16-methoxy-2,3-dihydrotabersonine = S-adenosyl-L-homocysteine + deacetoxyvindoline summerization of biosynthetic pathway of vindoline Catharanthus roseus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Catharanthus roseus

Storage Stability

Storage Stability Organism
-20°C, in absence of CHAPS, 4 days, 50% loss of activity Catharanthus roseus
-20°C, in absence of detergent by adding 25% glycerol, one week, 9% loss of activity Catharanthus roseus
-20°C, in presence of 0.6% CHAPS, 7 days, 50% loss of activity Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information hydrogenation of 2,3 double bond of tabersonine instead of hydration results in a 75% decrease in N-methylation, double bound present: no N-methylation, the presence of 6,7 double bound is a necessary structural requirement for N-methylation with membrane-bound enzyme, whereas the detergent-solubilized enzyme is also able to N-methylate the 6,7 anhydro analogue Catharanthus roseus ?
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 ?
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine high degree of specificity for the N-indole of 2,3-dihydro-3-hydroxytabersonine Catharanthus roseus S-adenosyl-L-homocysteine + deacetoxyvindoline
-
 ?
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine enzyme is involved in the biosynthesis of vindoline from tabersonine Catharanthus roseus S-adenosyl-L-homocysteine + deacetoxyvindoline
-
 ?