BRENDA - Enzyme Database
show all sequences of 2.1.1.99

Subcellular localization of enzymes involved in indole alkaloid biosynthesis in Catharanthus roseus

De Luca, V.; Cutler, A.J.; Plant Physiol. 85, 1099-1102 (1987)

Data extracted from this reference:

General Stability
General Stability
Organism
reducing agent, e.g. DTT, necessary for the retention of activity after breakage of thylakoid membranes of chloroplasts
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
associated with thylakoids
Catharanthus roseus
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
Catharanthus roseus
enzyme is involved in the biosynthesis of vindoline from tabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
-
Catharanthus roseus
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
additional information
optimal pH for solubilization: 7.5
Catharanthus roseus
Organism
Organism
UniProt
Commentary
Textmining
Catharanthus roseus
-
-
-
Oxidation Stability
Oxidation Stability
Organism
labile to O2, DTT or 2-mercaptoethanol stabilizes
Catharanthus roseus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
-
485690
Catharanthus roseus
S-adenosyl-L-homocysteine + deacetoxyvindoline
i.e. N(1)-methyl-16-methoxy-2,3-dihydro-3-hydroxytabersonine
485690
Catharanthus roseus
?
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
enzyme is involved in the biosynthesis of vindoline from tabersonine
485690
Catharanthus roseus
S-adenosyl-L-homocysteine + deacetoxyvindoline
-
485690
Catharanthus roseus
?
General Stability (protein specific)
General Stability
Organism
reducing agent, e.g. DTT, necessary for the retention of activity after breakage of thylakoid membranes of chloroplasts
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
associated with thylakoids
Catharanthus roseus
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
Catharanthus roseus
enzyme is involved in the biosynthesis of vindoline from tabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
-
Catharanthus roseus
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
additional information
optimal pH for solubilization: 7.5
Catharanthus roseus
Oxidation Stability (protein specific)
Oxidation Stability
Organism
labile to O2, DTT or 2-mercaptoethanol stabilizes
Catharanthus roseus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
-
485690
Catharanthus roseus
S-adenosyl-L-homocysteine + deacetoxyvindoline
i.e. N(1)-methyl-16-methoxy-2,3-dihydro-3-hydroxytabersonine
485690
Catharanthus roseus
?
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
enzyme is involved in the biosynthesis of vindoline from tabersonine
485690
Catharanthus roseus
S-adenosyl-L-homocysteine + deacetoxyvindoline
-
485690
Catharanthus roseus
?
Other publictions for EC 2.1.1.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485692
Dethier
-
Partial purification of an N-m ...
Catharanthus roseus
Phytochemistry
32
673-678
1993
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485690
De Luca
Subcellular localization of en ...
Catharanthus roseus
Plant Physiol.
85
1099-1102
1987
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485691
De Luca
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Biosynthesis of indole alkaloi ...
Catharanthus roseus
J. Plant Physiol.
125
147-156
1986
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