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Literature summary for 2.1.1.97 extracted from
- Actinomycin synthesis in Streptomyces antibioticus (1988), J. Biol. Chem., 263, 4602-4606.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Streptomyces antibioticus |  |
| Na2EDTA | - |
Streptomyces antibioticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
S-adenosyl-L-methionine | - |
Streptomyces antibioticus | |
| 0.64 | - |
3-Hydroxyanthranilate | - |
Streptomyces antibioticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
1 * 36000, SDS-PAGE | Streptomyces antibioticus |
| 37000 | - |
gel filtration, chromatography in 0.5 M NaCl | Streptomyces antibioticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 3-hydroxyanthranilate | Streptomyces antibioticus | involved in the biosynthesis of the antibiotic actinomycin | S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces antibioticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces antibioticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Streptomyces antibioticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.052 | - |
- |
Streptomyces antibioticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 5% glycerol, 50 mM sodium phosphate, pH 7.0, 10 mM Na2EDTA, 5 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, quite stable, can be frozen and thawed several times without appreciable loss of activity | Streptomyces antibioticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 3-hydroxyanthranilate | involved in the biosynthesis of the antibiotic actinomycin | Streptomyces antibioticus | S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
| S-adenosyl-L-methionine + 3-hydroxyanthranilic acid | - |
Streptomyces antibioticus | S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Streptomyces antibioticus |
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