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Literature summary for 2.1.1.97 extracted from

  • Jones, G.H.
    Actinomycin synthesis in Streptomyces antibioticus: Enzymatic conversion of 3-hydroxyanthranilic acid to 4-methyl-3-hydroxyanthranilic acid (1987), J. Bacteriol., 169, 5575-5578.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.97

Cloned(Commentary)

Cloned (Comment) Organism
gene for the 88000 Mr PHS subunit cloned Streptomyces antibioticus

Inhibitors

Inhibitors Comment Organism Structure
HEPES buffer
-
 Streptomyces antibioticus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane intracellular membrane associated Streptomyces antibioticus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000, SDS-PAGE Streptomyces antibioticus
37000
-
 gel filtration, chromatography in 0.5 M NaCl Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces sp.
-
 S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces anulatus
-
 S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces antibioticus involved in the biosynthesis of the antibiotic actinomycin S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces antibioticus
-
-
-
Streptomyces anulatus
-
-
-
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces antibioticus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Streptomyces antibioticus
-

Storage Stability

Storage Stability Organism
-70°C, 5% glycerol, 50 mM sodium phosphate, pH 7.0, 10 mM Na2EDTA, 5 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, quite stable, can be frozen and thawed several times without appreciable loss of activity Streptomyces antibioticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information does not catalyze the methylation of 3-hydroxy-DL-kynurenine Streptomyces antibioticus ?
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilate
-
 Streptomyces sp. S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilate
-
 Streptomyces anulatus S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilate involved in the biosynthesis of the antibiotic actinomycin Streptomyces antibioticus S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces sp. S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces antibioticus S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces anulatus S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Streptomyces antibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 sodium phosphate buffer Streptomyces antibioticus
7 8 Tris buffer Streptomyces antibioticus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Streptomyces antibioticus