Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
gamma-tocopherol | in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.0028 | - |
delta-tocopherol | in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.0087 | - |
S-adenosyl-L-methionine | in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33971 | - |
1 * 33971, calculated from amino acid sequence | Chlamydomonas reinhardtii |
37000 | - |
gel filtration | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | Q5CB51 | - |
- |
Chlamydomonas reinhardtii 6145c | Q5CB51 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-agarose column chromatography | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + delta-tocopherol | the enzyme has a similar affinity for gamma- and delta-tocopherol | Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + beta-tocopherol | - |
? | |
S-adenosyl-L-methionine + delta-tocopherol | the enzyme has a similar affinity for gamma- and delta-tocopherol | Chlamydomonas reinhardtii 6145c | S-adenosyl-L-homocysteine + beta-tocopherol | - |
? | |
S-adenosyl-L-methionine + gamma-tocopherol | the enzyme has a similar affinity for gamma- and delta-tocopherol | Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? | |
S-adenosyl-L-methionine + gamma-tocopherol | the enzyme has a similar affinity for gamma- and delta-tocopherol | Chlamydomonas reinhardtii 6145c | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Chlamydomonas reinhardtii |
monomer | 1 * 33971, calculated from amino acid sequence | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
gamma-TMT | - |
Chlamydomonas reinhardtii |
gamma-tocopherol methyltransferase | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
around 40°C | Chlamydomonas reinhardtii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | about 60% activity at 30°C, about 55% activity at 50°C | Chlamydomonas reinhardtii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | the enzyme is stable at 4°C, shows about 80% activity after 10 min at 30-35°C, about 60% activity after 10 min at 40°C, and is completely inactivated after 10 min at 45°C | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Chlamydomonas reinhardtii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 60% activity at pH 6.0, about 50% activity at pH 9.0 | Chlamydomonas reinhardtii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme is stable above pH 6.0 (more than 90% activity), even at alkaline pH 10.0 | Chlamydomonas reinhardtii |
Organism | Comment | Expression |
---|---|---|
Chlamydomonas reinhardtii | enzyme expression is downregulated (to 40%) by low temperature (10°C) | down |
Chlamydomonas reinhardtii | enzyme expression is strongly (about 80%) upregulated by high light | up |