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Literature summary for 2.1.1.95 extracted from

  • Galvez-Valdivieso, G.; Cardenosa, R.; Vera, J.M.; Pineda, M.; Aguilar, M.
    gamma-Tocopherol methyltransferase from the green alga Chlamydomonas reinhardtii: functional characterization and expression analysis (2011), Physiol. Plant., 143, 316-328.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
gamma-tocopherol in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication Chlamydomonas reinhardtii
0.0028
-
delta-tocopherol in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication Chlamydomonas reinhardtii
0.0087
-
S-adenosyl-L-methionine in 50 mM HEPES (pH 7.5), 5 mM dithiothreitol, temperature not specified in the publication Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33971
-
1 * 33971, calculated from amino acid sequence Chlamydomonas reinhardtii
37000
-
gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii Q5CB51
-
-
Chlamydomonas reinhardtii 6145c Q5CB51
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-agarose column chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + delta-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + beta-tocopherol
-
?
S-adenosyl-L-methionine + delta-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii 6145c S-adenosyl-L-homocysteine + beta-tocopherol
-
?
S-adenosyl-L-methionine + gamma-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + alpha-tocopherol
-
?
S-adenosyl-L-methionine + gamma-tocopherol the enzyme has a similar affinity for gamma- and delta-tocopherol Chlamydomonas reinhardtii 6145c S-adenosyl-L-homocysteine + alpha-tocopherol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Chlamydomonas reinhardtii
monomer 1 * 33971, calculated from amino acid sequence Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
gamma-TMT
-
Chlamydomonas reinhardtii
gamma-tocopherol methyltransferase
-
Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
around 40°C Chlamydomonas reinhardtii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 about 60% activity at 30°C, about 55% activity at 50°C Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 45 the enzyme is stable at 4°C, shows about 80% activity after 10 min at 30-35°C, about 60% activity after 10 min at 40°C, and is completely inactivated after 10 min at 45°C Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 60% activity at pH 6.0, about 50% activity at pH 9.0 Chlamydomonas reinhardtii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 the enzyme is stable above pH 6.0 (more than 90% activity), even at alkaline pH 10.0 Chlamydomonas reinhardtii

Expression

Organism Comment Expression
Chlamydomonas reinhardtii enzyme expression is downregulated (to 40%) by low temperature (10°C) down
Chlamydomonas reinhardtii enzyme expression is strongly (about 80%) upregulated by high light up