Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | slight stimulation | Euglena gracilis |
General Stability | Organism |
---|---|
very labile in absence of MnCl2, complete inactivation within 36 h at 4°C, 50% loss of activity after 7 h at 4°C, stabilized by addition of 1 mM MnCl2, only 15% loss of activity after 90 h at 4°C | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Euglena gracilis | |
Fe2+ | - |
Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
S-adenosyl-L-methionine | - |
Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Euglena gracilis | 9507 | - |
membrane | loosely bound to outer chloroplast membrane | Euglena gracilis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect with Mg2+, Ca2+, K+ and Na+ | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + gamma-tocopherol | Euglena gracilis | biosynthesis of alpha-tocopherol | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
purified enzyme | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + beta-tocopherol | 70% as effective as gamma-tocopherol | Euglena gracilis | ? | - |
? | |
S-adenosyl-L-methionine + delta-tocopherol | 35% as effective as gamma-tocopherol | Euglena gracilis | S-adenosyl-L-homocysteine + beta-tocopherol | - |
? | |
S-adenosyl-L-methionine + gamma-tocopherol | - |
Euglena gracilis | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? | |
S-adenosyl-L-methionine + gamma-tocopherol | biosynthesis of alpha-tocopherol | Euglena gracilis | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Euglena gracilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Euglena gracilis |