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Literature summary for 2.1.1.94 extracted from
- Application of carborundum abrasion for investigating the leaf epidermis: molecular cloning of Catharanthus roseus 16-hydroxytabersonine-16-O-methyltransferase (2008), Plant J., 53, 225-236.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 16-hydroxytabersonine-16-O-methyltransferase is isolated and cloned as a His-tagged fusion protein in Escherichia coli | Catharanthus roseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | product inhibition | Catharanthus roseus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
16-hydroxytabersonine | - |
Catharanthus roseus | |
| 0.0217 | - |
S-adenosyl-L-methionine | - |
Catharanthus roseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Catharanthus roseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | B0EXJ8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using PD-10 column chromatography, Sephadex G150 column chromatography, adenosine-agarose affinity resin and Mono Q anion exchange column chromatography | Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | predominatly expressed in leaf epidermis | Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000417 | - |
16-hydroxytabersonine as a substrate | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 16-hydroxytabersonine | 16OMT is highly specific for its alkaloid substrate 16-hydroxytabersonine | Catharanthus roseus | S-adenosyl-L-homocysteine + 16-methoxytabersonine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 16-hydroxytabersonine-16-O-methyltransferase | - |
Catharanthus roseus |
| 16OMT | - |
Catharanthus roseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Catharanthus roseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Catharanthus roseus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
S-adenosyl-L-homocysteine | product inhibition | Catharanthus roseus | |
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