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Literature summary for 2.1.1.9 extracted from
- Isolation and characterization of a gene encoding a S-adenosyl-L-methionine-dependent halide/thiol methyltransferase (HTMT) from the marine diatom Phaeodactylum tricornutum: Biogenic mechanism of CH3I emissions in oceans (2011), Phytochemistry, 72, 337-343.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged protein expressed in Escherichia coli BL21(DE3), together with chaperone-coding genes (plasmid pG-KJE8) | Phaeodactylum tricornutum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.9 | - |
thiocyanate | pH 7.0, 30°C | Phaeodactylum tricornutum |  |
| 9.9 | - |
SH- | pH 7.0, 30°C | Phaeodactylum tricornutum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration, His-tagged protein | Phaeodactylum tricornutum |
| 34000 | - |
1 * 34000, SDS-PAGE, His-tagged protein | Phaeodactylum tricornutum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaeodactylum tricornutum | B7FWR0 | - |
- |
| Phaeodactylum tricornutum CCAP 1055/1 | B7FWR0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Phaeodactylum tricornutum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1043 | - |
substrate iodometane, pH 7.0, 30°C | Phaeodactylum tricornutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + thiocyanate | - |
Phaeodactylum tricornutum | S-adenosyl-L-homocysteine + methyl thiocyanate | - |
? | |
| S-adenosyl-L-methionine + thiocyanate | - |
Phaeodactylum tricornutum CCAP 1055/1 | S-adenosyl-L-homocysteine + methyl thiocyanate | - |
? | |
| SH- + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum | CH3SH + S-adenosyl-L-homocysteine | - |
? | |
| SH- + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum CCAP 1055/1 | CH3SH + S-adenosyl-L-homocysteine | - |
? | |
| thiocyanate + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum | methyl thiocyanate + S-adenosyl-L-homocysteine | - |
? | |
| thiocyanate + S-adenosyl-L-methionine | - |
Phaeodactylum tricornutum CCAP 1055/1 | methyl thiocyanate + S-adenosyl-L-homocysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE, His-tagged protein | Phaeodactylum tricornutum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S-adenosyl-L-methionine-dependent halide/thiol methyltransferase | - |
Phaeodactylum tricornutum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0102 | - |
thiocyanate | pH 7.0, 30°C | Phaeodactylum tricornutum | |
| 0.0381 | - |
SH- | pH 7.0, 30°C | Phaeodactylum tricornutum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
50% activity at pH 6.0 or pH 8.5 | Phaeodactylum tricornutum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00129 | - |
SCN- | pH 7.0, 30°C | Phaeodactylum tricornutum | |
| 0.00385 | - |
SH- | pH 7.0, 30°C | Phaeodactylum tricornutum | |
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