Application | Comment | Organism |
---|---|---|
additional information | a few changes in key residues are sufficient to convert an OMT into a S-methyltransferase | Catharanthus roseus |
Cloned (Comment) | Organism |
---|---|
subcloned for expression of a His-tagged recombinant protein in Escherichia coli | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by Mg2+ | Catharanthus roseus | |
Zn2+ | ca. 90% inhibition at 1 mM, at low concentrations the effect is substrate-dependent | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
furfuryl thiol | - |
Catharanthus roseus | |
0.096 | - |
S-adenosyl-L-methionine | when assayed with benzene thiol and furfuryl thiol | Catharanthus roseus | |
0.218 | - |
benzene thiol | - |
Catharanthus roseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to near homogeneity by Ni-chelation affinity chromatography | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-hexanediol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2-mercaptoacetic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
2-mercaptopropanoic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
3-mercaptohexan-1-ol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
3-mercaptopropanoic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
6-mercaptohexan-1-ol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
benzene thiol + S-adenosyl-L-methionine | - |
Catharanthus roseus | methyl phenyl sulfide + S-adenosyl-L-homocysteine | - |
? | |
dithiothreitol + S-adenosyl-L-methionine | - |
Catharanthus roseus | ? + S-adenosyl-L-homocysteine | - |
? | |
ethanethioic S-acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | S-methylethanethioate + S-adenosyl-L-homocysteine | - |
? | |
furfuryl thiol + S-adenosyl-L-methionine | - |
Catharanthus roseus | 2-[(methylsulfanyl)methyl]furan + S-adenosyl-L-homocysteine | - |
? | |
guaiacol + S-adenosyl-L-methionine | - |
Catharanthus roseus | 1,2-dimethoxybenzene + S-adenosyl-L-homocysteine | - |
? | |
additional information | no activity with flavonoids and hydroxyl group-containing compounds, inactive with Na2S and 3-mercaptobenzoic acid, presence of carboxyl groups abolishes CrSMT1 activity, threitol, furfuryl alcohol, benzoic acid, 1,3-propandiol, 1,6-hexanediol and pyrocatechol possess negligible activity | Catharanthus roseus | ? | - |
? | |
phenol + S-adenosyl-L-methionine | - |
Catharanthus roseus | methoxybenzene + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CrSMT1 | - |
Catharanthus roseus |
S-methyltransferase 1 | - |
Catharanthus roseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
with furfuryl thiol as substrate | Catharanthus roseus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | with furfuryl thiol as substrate | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.3 | with furfuryl thiol as substrate | Catharanthus roseus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | with furfuryl thiol as substrate | Catharanthus roseus |