Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Populus sp. |
Protein Variants | Comment | Organism |
---|---|---|
D257G | the mutant methylates the 3-hydroxyl group of flavonols in addition to the 7-hydrdoxyl group | Populus sp. |
L101M | the mutant shows wild type reactivity with kaempferol and quercetin | Populus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0298 | - |
quercetin | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
0.0324 | - |
quercetin | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
0.0324 | - |
kaempferol | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
0.0378 | - |
kaempferol | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Populus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST affinity column chromatography | Populus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + kaempferol | - |
Populus sp. | 7-O-methylkaempferol | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Populus sp. | 7-O-methylquercetin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Populus sp. |
Synonyms | Comment | Organism |
---|---|---|
flavone 7-O-methyltransferase | - |
Populus sp. |
MT7 | - |
Populus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
quercetin | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
0.064 | - |
kaempferol | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
0.07 | - |
kaempferol | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
0.071 | - |
quercetin | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
kaempferol | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
19 | - |
quercetin | mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
19 | - |
kaempferol | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. | |
24 | - |
quercetin | wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C | Populus sp. |