General Stability | Organism |
---|---|
loss of activity during chromatofocusing | Chrysosplenium americanum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Chrysosplenium americanum | |
p-chloromercuribenzoate | - |
Chrysosplenium americanum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Chrysosplenium americanum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone | Chrysosplenium americanum | involved with EC 2.1.1.76 and 2.1.1.83 in the methylation of quercetin | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chrysosplenium americanum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
loss of activity during chromatofocusing possibly due to instability of the enzyme in the purified form or to the loss of some factors required for activity | Chrysosplenium americanum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Chrysosplenium americanum | - |
Storage Stability | Organism |
---|---|
-15°C, 200 mM potassium phosphate buffer, pH 8, 20% v/v glycerol, 5 mM dithiothreitol, several months, no loss of activity | Chrysosplenium americanum |
4°C, 4-6 weeks, 80% loss of activity | Chrysosplenium americanum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone | trivial name 3-methylquercetin, specific for 3-methylquercetin | Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin | ? | |
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone | involved with EC 2.1.1.76 and 2.1.1.83 in the methylation of quercetin | Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Chrysosplenium americanum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.5 | approx. 50% of maximal activity at pH 7.2 and 9.5 | Chrysosplenium americanum |