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Literature summary for 2.1.1.80 extracted from

  • Garcia-Fontana, C.; Reyes-Darias, J.A.; Munoz-Martinez, F.; Alfonso, C.; Morel, B.; Ramos, J.L.; Krell, T.
    High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation (2013), J. Biol. Chem., 288, 18987-18999.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida
expressed in Escherichia coli C41(DE3) cells Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32247
-
 1 * 32247, isoform CheR2, calculated from amino acid sequence Pseudomonas putida
38600
-
 1 * 38600, isoform CheR2, sedimentation equilibrium ultracentrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate Pseudomonas putida substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate Pseudomonas putida KT 2240 substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate Pseudomonas putida substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate Pseudomonas putida KT 2240 substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88ER1
-
-
Pseudomonas putida Q88GG4
-
-
Pseudomonas putida Q88MS8
-
-
Pseudomonas putida KT 2240 Q88ER1
-
-
Pseudomonas putida KT 2240 Q88GG4
-
-
Pseudomonas putida KT 2240 Q88MS8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Pseudomonas putida
HisTrap column chromatography, and gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with chemotaxis receptors McpS and chemotaxis receptors McpT Pseudomonas putida ?
-
 ?
additional information no activity with chemotaxis receptors McpS and chemotaxis receptors McpT Pseudomonas putida KT 2240 ?
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate substrate for isoform CheR2 only Pseudomonas putida S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate substrate for isoform CheR2 only Pseudomonas putida KT 2240 S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate substrate for isoform CheR2 only Pseudomonas putida S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate substrate for isoform CheR2 only Pseudomonas putida KT 2240 S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32247, isoform CheR2, calculated from amino acid sequence Pseudomonas putida
monomer 1 * 38600, isoform CheR2, sedimentation equilibrium ultracentrifugation Pseudomonas putida

Synonyms

Synonyms Comment Organism
CheR1 isoform Pseudomonas putida
CheR2 isoform Pseudomonas putida
CheR3 isoform Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
 isoform CheR1 has a melting temperature of 44°C Pseudomonas putida
48
-
 isoform CheR2 has a melting temperature of 48°C Pseudomonas putida
49
-
 isoform CheR3 has a melting temperature of 49°C Pseudomonas putida

General Information

General Information Comment Organism
physiological function isoform CheR1 is required for efficient biofilm formation Pseudomonas putida
physiological function isoform CheR2 is essential for chemotaxis Pseudomonas putida