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Literature summary for 2.1.1.80 extracted from

  • Kim, S.
    S-Adenosylmethionine: protein-carboxyl O-methyltransferase (protein methylase II) (1984), Methods Enzymol., 106, 295-309.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
A9145C S-adenosylmethionine analog, antifungal antibiotic Bos taurus
A9145C S-adenosylmethionine analog, antifungal antibiotic Escherichia coli
Ca2+ MCP methyltransferase I Bacillus subtilis
Ca2+ MCP methyltransferase I Escherichia coli
Ca2+ MCP methyltransferase I Salmonella enterica subsp. enterica serovar Typhimurium
p-chloromercuribenzoate 0.4 mM, 40% inhibition, can be reversed by 12 mM 2-mercaptoethanol Bos taurus
S-adenosyl-L-homocysteine D-isomer is inactive Bacillus subtilis
S-adenosyl-L-homocysteine
-
Bos taurus
S-adenosyl-L-homocysteine D-isomer is inactive Escherichia coli
S-adenosyl-L-homocysteine
-
Homo sapiens
S-adenosyl-L-homocysteine D-isomer is inactive Salmonella enterica subsp. enterica serovar Typhimurium
sinefungin S-adenosylmethionine analog, antifungal antibiotic Bos taurus
sinefungin S-adenosylmethionine analog, antifungal antibiotic Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00087
-
S-adenosyl-L-methionine thymus enzyme Bos taurus
0.002
-
S-adenosyl-L-methionine MCP methyltransferase I Bacillus subtilis
0.005
-
S-adenosyl-L-methionine MCP methyltransferase II Bacillus subtilis
0.0077
-
[follicle-stimulating hormone]-L-glutamate
-
Bos taurus
0.01
-
S-adenosyl-L-methionine
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.046
-
[gamma-globulin]-L-glutamate
-
Bos taurus
0.1
-
[histone]-L-glutamate
-
Bos taurus
0.34
-
[pancreatic ribonuclease]-L-glutamate
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I Salmonella enterica subsp. enterica serovar Typhimurium
Ca2+ Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I Bacillus subtilis
Ca2+ Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I Escherichia coli
Ca2+ Mg2+ and Ca2+ activate MCP methyltransferase II Bacillus subtilis
Mg2+ Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I Bacillus subtilis
Mg2+ Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I Escherichia coli
Mg2+ Mg2+ and Ca2+ activate MCP methyltransferase II Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
gel filtration Mammalia
30000
-
gel filtration, SDS-PAGE Bacillus subtilis
44000
-
MCP methyltransferase I Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
MCP methyltransferase I and II
-
Bos taurus
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Mammalia
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-75, SAH-Sepharose, Sephadex G-75 Homo sapiens
brain enzyme, pH 5.1 treatment, ammonium sulfate, SAH-Sepharose, Sephadex G-100 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-
thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0043
-
thymus enzyme Bos taurus
0.027
-
erythrocyte enzyme Homo sapiens
0.03
-
brain enzyme Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 20-50% glycerol, 5 mM sodium borate, 5 mM EDTA, 2.4 mM 2-mercaptoethanol, pH 9.3, 1 year, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + protein L-glutamate S-adenosyl-L-ethionine shows 2.4% donor efficiency compared to S-adenosyl-L-methionine Salmonella enterica subsp. enterica serovar Typhimurium S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + protein L-glutamate S-adenosyl-L-ethionine shows 2.4% donor efficiency compared to S-adenosyl-L-methionine Bacillus subtilis S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + protein L-glutamate S-adenosyl-L-ethionine shows 2.4% donor efficiency compared to S-adenosyl-L-methionine Escherichia coli S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + protein L-glutamate highly specific for S-adenosylmethionine as methyl donor Salmonella enterica subsp. enterica serovar Typhimurium S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + protein L-glutamate highly specific for S-adenosylmethionine as methyl donor Bacillus subtilis S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + protein L-glutamate highly specific for S-adenosylmethionine as methyl donor Escherichia coli S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + [follicle-stimulating hormone]-L-glutamate
-
Bos taurus S-adenosyl-L-homocysteine + [follicle-stimulating hormone]-L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + [gamma-globulin]-L-glutamate
-
Bos taurus S-adenosyl-L-homocysteine + [gamma-globulin]-L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + [histone]-L-glutamate
-
Bos taurus S-adenosyl-L-homocysteine + [histone]-L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + [pancreatic ribonuclease]-L-glutamate
-
Bos taurus S-adenosyl-L-homocysteine + [pancreatic ribonuclease]-L-glutamate methyl ester
-
?
S-adenosyl-L-methionine + [protein]-L-glutamate
-
Bos taurus S-adenosyl-L-homocysteine + [protein]-L-glutamate methyl ester
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
S-adenosyl-L-homocysteine
-
Bacillus subtilis
0.0004
-
A9145C
-
Bos taurus
0.0009
-
S-adenosyl-L-homocysteine
-
Homo sapiens
0.0009
-
S-adenosyl-L-homocysteine
-
Bos taurus
0.0026
-
sinefungin
-
Bos taurus