Inhibitors | Comment | Organism | Structure |
---|---|---|---|
A9145C | S-adenosylmethionine analog, antifungal antibiotic | Bos taurus | |
A9145C | S-adenosylmethionine analog, antifungal antibiotic | Escherichia coli | |
Ca2+ | MCP methyltransferase I | Bacillus subtilis | |
Ca2+ | MCP methyltransferase I | Escherichia coli | |
Ca2+ | MCP methyltransferase I | Salmonella enterica subsp. enterica serovar Typhimurium | |
p-chloromercuribenzoate | 0.4 mM, 40% inhibition, can be reversed by 12 mM 2-mercaptoethanol | Bos taurus | |
S-adenosyl-L-homocysteine | D-isomer is inactive | Bacillus subtilis | |
S-adenosyl-L-homocysteine | - |
Bos taurus | |
S-adenosyl-L-homocysteine | D-isomer is inactive | Escherichia coli | |
S-adenosyl-L-homocysteine | - |
Homo sapiens | |
S-adenosyl-L-homocysteine | D-isomer is inactive | Salmonella enterica subsp. enterica serovar Typhimurium | |
sinefungin | S-adenosylmethionine analog, antifungal antibiotic | Bos taurus | |
sinefungin | S-adenosylmethionine analog, antifungal antibiotic | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00087 | - |
S-adenosyl-L-methionine | thymus enzyme | Bos taurus | |
0.002 | - |
S-adenosyl-L-methionine | MCP methyltransferase I | Bacillus subtilis | |
0.005 | - |
S-adenosyl-L-methionine | MCP methyltransferase II | Bacillus subtilis | |
0.0077 | - |
[follicle-stimulating hormone]-L-glutamate | - |
Bos taurus | |
0.01 | - |
S-adenosyl-L-methionine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.046 | - |
[gamma-globulin]-L-glutamate | - |
Bos taurus | |
0.1 | - |
[histone]-L-glutamate | - |
Bos taurus | |
0.34 | - |
[pancreatic ribonuclease]-L-glutamate | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ca2+ | Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I | Bacillus subtilis | |
Ca2+ | Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I | Escherichia coli | |
Ca2+ | Mg2+ and Ca2+ activate MCP methyltransferase II | Bacillus subtilis | |
Mg2+ | Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I | Bacillus subtilis | |
Mg2+ | Ca2+, Mn2+, Cu2+, Zn2+, Co2+, Fe2+, Fe3+ and Mg2+ at 2 mM have no effect on enzyme activity of methyltransferase I | Escherichia coli | |
Mg2+ | Mg2+ and Ca2+ activate MCP methyltransferase II | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
gel filtration | Mammalia |
30000 | - |
gel filtration, SDS-PAGE | Bacillus subtilis |
44000 | - |
MCP methyltransferase I | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
MCP methyltransferase I and II | - |
Bos taurus | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Mammalia | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-75, SAH-Sepharose, Sephadex G-75 | Homo sapiens |
brain enzyme, pH 5.1 treatment, ammonium sulfate, SAH-Sepharose, Sephadex G-100 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0043 | - |
thymus enzyme | Bos taurus |
0.027 | - |
erythrocyte enzyme | Homo sapiens |
0.03 | - |
brain enzyme | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, 20-50% glycerol, 5 mM sodium borate, 5 mM EDTA, 2.4 mM 2-mercaptoethanol, pH 9.3, 1 year, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + protein L-glutamate | S-adenosyl-L-ethionine shows 2.4% donor efficiency compared to S-adenosyl-L-methionine | Salmonella enterica subsp. enterica serovar Typhimurium | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-glutamate | S-adenosyl-L-ethionine shows 2.4% donor efficiency compared to S-adenosyl-L-methionine | Bacillus subtilis | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-glutamate | S-adenosyl-L-ethionine shows 2.4% donor efficiency compared to S-adenosyl-L-methionine | Escherichia coli | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-glutamate | highly specific for S-adenosylmethionine as methyl donor | Salmonella enterica subsp. enterica serovar Typhimurium | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-glutamate | highly specific for S-adenosylmethionine as methyl donor | Bacillus subtilis | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + protein L-glutamate | highly specific for S-adenosylmethionine as methyl donor | Escherichia coli | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + [follicle-stimulating hormone]-L-glutamate | - |
Bos taurus | S-adenosyl-L-homocysteine + [follicle-stimulating hormone]-L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + [gamma-globulin]-L-glutamate | - |
Bos taurus | S-adenosyl-L-homocysteine + [gamma-globulin]-L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + [histone]-L-glutamate | - |
Bos taurus | S-adenosyl-L-homocysteine + [histone]-L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + [pancreatic ribonuclease]-L-glutamate | - |
Bos taurus | S-adenosyl-L-homocysteine + [pancreatic ribonuclease]-L-glutamate methyl ester | - |
? | |
S-adenosyl-L-methionine + [protein]-L-glutamate | - |
Bos taurus | S-adenosyl-L-homocysteine + [protein]-L-glutamate methyl ester | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
S-adenosyl-L-homocysteine | - |
Bacillus subtilis | |
0.0004 | - |
A9145C | - |
Bos taurus | |
0.0009 | - |
S-adenosyl-L-homocysteine | - |
Homo sapiens | |
0.0009 | - |
S-adenosyl-L-homocysteine | - |
Bos taurus | |
0.0026 | - |
sinefungin | - |
Bos taurus |