Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
S-adenosyl-L-methionine | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus subtilis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, Ca2+ is somewhat more effective than Mg2+, optimum: 5 mM Ca2+ | Bacillus subtilis | |
Mg2+ | activates, Ca2+ is somewhat more effective than Mg2+, optimum: 5 mM Ca2+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Biogel, CM-Biogel, ammonium sulfate, S-adenosylhomocystein affinity column | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0024 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
0°C, 0.33 mM dithiothreitol, 0.33 mg/ml bovine serum albumin, 1 week, no loss of activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + protein L-glutamate | membrane-bound methyl-accepting chemotaxis proteins | Bacillus subtilis | S-adenosyl-L-homocysteine + protein L-glutamate methyl ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | approx. 30% of maximal activity at pH 6.0, approx. 65% of maximal activity at pH 8.0 | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
S-adenosyl-L-homocysteine | - |
Bacillus subtilis |