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Literature summary for 2.1.1.77 extracted from

  • Kim, J.; Chen, B.; Bru, J.L.; Huynh, E.; Momen, M.; Aswad, D.W.
    New findings on SNP variants of human protein L-isoaspartyl methyltransferase that affect catalytic activity, thermal stability, and aggregation (2018), PLoS ONE, 13, e0198266 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
A150V the mutant shows 64% loss of activity compared to the wild type activity Homo sapiens
A65V the mutant shows 11% loss of activity compared to the wild type activity Homo sapiens
A7P the mutant shows 34% of wild type catalytic efficiency Homo sapiens
F72L the mutant shows 67% of wild type catalytic efficiency Homo sapiens
I58V the mutant shows 60% of wild type catalytic efficiency Homo sapiens
L191S the mutant shows 72% loss of activity compared to the wild type activity Homo sapiens
P174H the mutant shows 61% loss of activity compared to the wild type activity Homo sapiens
R36A the mutant shows near complete loss of activity (<1%) Homo sapiens
R36C the mutant shows near complete loss of activity (<1%) Homo sapiens
R36K the mutant shows near complete loss of activity (4.6%) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Homo sapiens
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + gamma-globulin
-
 Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + isoAsp-DSIP
-
 Homo sapiens S-adenosyl-L-homocysteine + isoAsp-alpha-methyl ester-DSIP
-
 ?
S-adenosyl-L-methionine + protein L-isoaspartate
-
 Homo sapiens S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
PCMT1
-
 Homo sapiens
PIMT
-
 Homo sapiens
protein L-isoaspartyl methyltransferase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57.6
-
 melting temperature Homo sapiens