Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + protein L-isoaspartate | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + protein L-isoaspartate | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
S-adenosyl-L-methionine + VYP-(L-iso-Asp)-HA | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + VYP-(L-iso-Asp-alpha-methyl ester)-HA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PIMT | - |
Arabidopsis thaliana |
PIMT1 | - |
Arabidopsis thaliana |
PIMT2 | - |
Arabidopsis thaliana |
protein L-isoaspartyl methyltransferase | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | heat and oxidative stress treatments strongly induce PIMT1 and PIMT2 transcript expression | up |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme activity is important to reduce stress-induced iso-Asp accumulation which otherwise affects plant growth and development and survivability under stress conditions. The enzyme restricts reactive oxygen species accumulation and lipid peroxidation during heat and oxidative stresses, by protecting functionality of antioxidant enzymes, and improves stress tolerance. Transgenic plants expressing either isoform PIMT1 or PIMT2 show increased stress tolerance, and survive better than the wild type plants under heat (37°C) and oxidative stress (H2O2) conditions | Arabidopsis thaliana |