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Literature summary for 2.1.1.77 extracted from

  • Ghosh, S.; Kamble, N.U.; Verma, P.; Salvi, P.; Petla, B.P.; Roy, S.; Rao, V.; Hazra, A.; Varshney, V.; Kaur, H.; Majee, M.
    Arabidopsis protein L-isoaspartyl methyltransferaserepairs isoaspartyl damage to antioxidant enzymes and increases heat and oxidative stress tolerance (2019), J. Biol. Chem., 295, 783-799 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + VYP-(L-iso-Asp)-HA
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + VYP-(L-iso-Asp-alpha-methyl ester)-HA
-
?

Synonyms

Synonyms Comment Organism
PIMT
-
Arabidopsis thaliana
PIMT1
-
Arabidopsis thaliana
PIMT2
-
Arabidopsis thaliana
protein L-isoaspartyl methyltransferase
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana heat and oxidative stress treatments strongly induce PIMT1 and PIMT2 transcript expression up

General Information

General Information Comment Organism
physiological function enzyme activity is important to reduce stress-induced iso-Asp accumulation which otherwise affects plant growth and development and survivability under stress conditions. The enzyme restricts reactive oxygen species accumulation and lipid peroxidation during heat and oxidative stresses, by protecting functionality of antioxidant enzymes, and improves stress tolerance. Transgenic plants expressing either isoform PIMT1 or PIMT2 show increased stress tolerance, and survive better than the wild type plants under heat (37°C) and oxidative stress (H2O2) conditions Arabidopsis thaliana