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Literature summary for 2.1.1.77 extracted from

  • Yin, L.; Harwood, C.S.
    Functional divergence of annotated L-isoaspartate O-methyltransferases in an alpha-proteobacterium (2019), J. Biol. Chem., 294, 2854-2861 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner cells Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 KASA-isoD-LAKY isoform RPA0376, at pH 6.4 and 30°C Rhodopseudomonas palustris
0.03
-
 VYR-isoD-RR isoform RPA0376, at pH 6.4 and 30°C Rhodopseudomonas palustris
0.034
-
 VYP-isoD-HA isoform RPA2838, at pH 6.4 and 30°C Rhodopseudomonas palustris
0.045
-
 VYP-isoD-HA isoform RPA0376, at pH 6.4 and 30°C Rhodopseudomonas palustris
0.12
-
 KASA-isoD-LAKY isoform RPA2838, at pH 6.4 and 30°C Rhodopseudomonas palustris
0.4
-
 VYR-isoD-RR isoform RPA2838, at pH 6.4 and 30°C Rhodopseudomonas palustris
2.6
-
 YVS-isoD-GHG isoform RPA0376, at pH 6.4 and 30°C Rhodopseudomonas palustris
3.1
-
 YVS-isoD-GHG isoform RPA2838, at pH 6.4 and 30°C Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Rhodopseudomonas palustris
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ir

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Pur resin column chromatography Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + KASA-isoD-LAKY
-
 Rhodopseudomonas palustris S-adenosyl-L-homocysteine + KASA-isoD alpha methyl ester-LAKY
-
 ?
S-adenosyl-L-methionine + protein L-isoaspartate
-
 Rhodopseudomonas palustris S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ir
S-adenosyl-L-methionine + VYP-isoD-HA
-
 Rhodopseudomonas palustris S-adenosyl-L-homocysteine + VYP-isoD alpha methyl ester-HA
-
 ?
S-adenosyl-L-methionine + VYR-isoD-RR
-
 Rhodopseudomonas palustris S-adenosyl-L-homocysteine + VYR-isoD alpha methyl ester-RR
-
 ?
S-adenosyl-L-methionine + YVS-isoD-GHG
-
 Rhodopseudomonas palustris S-adenosyl-L-homocysteine + YVS-isoD alpha methyl ester-GHG
-
 ?

Synonyms

Synonyms Comment Organism
L-isoaspartate O-methyltransferase
-
 Rhodopseudomonas palustris
PIMT
-
 Rhodopseudomonas palustris
protein L-isoaspartate O-methyltransferase
-
 Rhodopseudomonas palustris
RPA0376 isoform Rhodopseudomonas palustris
RPA2838 isoform Rhodopseudomonas palustris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoform RPA0376 Rhodopseudomonas palustris
50
-
 isoform RPA2838, the enzyme activity increases sharply when the temperature is increased from 30 to 50°C. A further increase to 60 °C results in a loss of activity Rhodopseudomonas palustris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 isoform RPA0376, the enzyme has optimal activity over a wide temperature range from 30 to 50°C. Enzyme activity is about 50% lower at 20°C and 90% lower at 60°C Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 9.4 isoform RPA2838 shows similar activities over a pH range from pH 5.4 to 9.4 Rhodopseudomonas palustris
6.4 7.5 isoform RPA0376 shows similar activities over a pH range from pH 6.4 to 7.5 Rhodopseudomonas palustris