Literature summary for 2.1.1.77 extracted from

Chatterjee, T.; Pal, A.; Chakravarty, D.; Dey, S.; Saha, R.P.; Chakrabarti, P.
Protein L-isoaspartyl-O-methyltransferase of Vibrio cholerae: interaction with cofactors and effect of osmolytes on unfolding (2013), Biochimie, 95, 912-921.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	1 * 26000, SDS-PAGE	Vibrio cholerae serotype O1
26420	-	1 * 26420, mass spectrometry	Vibrio cholerae serotype O1
26486	-	1 * 26486, calculated from amino acid sequence	Vibrio cholerae serotype O1
26600	-	gel filtration	Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + protein D-aspartate	Vibrio cholerae serotype O1	-	S-adenosyl-L-homocysteine + protein D-aspartate methyl ester	-	?
S-adenosyl-L-methionine + protein L-isoaspartate	Vibrio cholerae serotype O1	-	S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + protein D-aspartate	-	Vibrio cholerae serotype O1	S-adenosyl-L-homocysteine + protein D-aspartate methyl ester	-	?
S-adenosyl-L-methionine + protein L-isoaspartate	-	Vibrio cholerae serotype O1	S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 26000, SDS-PAGE	Vibrio cholerae serotype O1
monomer	1 * 26420, mass spectrometry	Vibrio cholerae serotype O1
monomer	1 * 26486, calculated from amino acid sequence	Vibrio cholerae serotype O1

Synonyms

Synonyms	Comment	Organism
PIMT	-	Vibrio cholerae serotype O1
protein L-isoaspartyl-O-methyltransferase	-	Vibrio cholerae serotype O1

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Release 2023.1 (January 2023)