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Literature summary for 2.1.1.77 extracted from

  • Thapar, N.; Clarke, S.
    Expression, Purification, and Characterization of the Protein Repair L-Isoaspartyl Methyltransferase from Arabidopsis thaliana (2000), Protein Expr. Purif., 20, 237-251.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens
-
Rattus norvegicus
-
Caenorhabditis elegans
-
Thermotoga maritima
cDNA isolation Triticum aestivum
cDNA isolation, pcm gene cloned and expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00029
-
VYP (isoAsp) HA
-
Homo sapiens
0.00052
-
KASA (isoD) LAKY
-
Homo sapiens
0.0019
-
S-adenosyl-L-methionine
-
Thermotoga maritima
0.0022
-
S-adenosyl-L-methionine
-
Homo sapiens
0.0028
-
KASA (isoAsp) LAKY
-
Thermotoga maritima
0.0031
-
S-adenosyl-L-methionine
-
Caenorhabditis elegans
0.0041
-
VYP (isoAsp) HA
-
Thermotoga maritima
0.006
-
S-adenosyl-L-methionine
-
Arabidopsis thaliana
0.00912
-
KASA (isoAsp) LAKY
-
Caenorhabditis elegans
0.0118
-
VYP (isoAsp) HA
-
Escherichia coli
0.0127
-
KASA (isoAsp) LAKY
-
Triticum aestivum
0.0194
-
VYP (isoD) HA
-
Caenorhabditis elegans
0.03
-
ovalbumin
-
Homo sapiens
0.043
-
VYP-L-isoAsp-HA
-
Zea mays
0.0506
-
KASA (isoAsp) LAKY
-
Escherichia coli
0.0517
-
VYP-L-isoAsp-HA
-
Triticum aestivum
0.08
-
KASA-L-isoAsp-LAKY
-
Arabidopsis thaliana
0.092
-
KASA-L-isoAsp-LAKY
-
Zea mays
0.31
-
VYP-L-isoAsp-HA
-
Arabidopsis thaliana
0.326
-
ovalbumin
-
Thermotoga maritima
0.727
-
ovalbumin
-
Escherichia coli
1.2
-
ovalbumin native enzyme Arabidopsis thaliana
2.66
-
ovalbumin
-
Zea mays
4.95
-
ovalbumin
-
Arabidopsis thaliana
5
-
ovalbumin
-
Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-
cytoplasm
-
Xenopus laevis 5737
-
cytosol
-
Escherichia coli 5829
-
cytosol
-
Homo sapiens 5829
-
cytosol
-
Rattus norvegicus 5829
-
cytosol
-
Arabidopsis thaliana 5829
-
cytosol
-
Caenorhabditis elegans 5829
-
cytosol
-
Thermotoga maritima 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24600
-
amino acid sequence Arabidopsis thaliana
28000
-
SDS-PAGE Homo sapiens
28000
-
SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Drosophila melanogaster
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Escherichia coli
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Homo sapiens
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Rattus norvegicus
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Triticum aestivum
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Zea mays
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Xenopus laevis
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Caenorhabditis elegans
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Solanum lycopersicum
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Oryza sativa
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Thermotoga maritima
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Arabidopsis thaliana repair enzyme, methylates abnormal L-isoaspartate residues in proteins which arise spontaneously as a result of aging S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
Thale cress
-
Caenorhabditis elegans
-
-
-
Drosophila melanogaster
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
human
-
Oryza sativa
-
rice
-
Rattus norvegicus
-
-
-
Solanum lycopersicum
-
tomato, Mill.
-
Thermotoga maritima
-
-
-
Triticum aestivum
-
wheat
-
Xenopus laevis
-
-
-
Zea mays
-
maize, corn
-

Purification (Commentary)

Purification (Comment) Organism
-
Triticum aestivum
recombinant protein Homo sapiens
recombinant protein Rattus norvegicus
recombinant protein Arabidopsis thaliana
recombinant protein Caenorhabditis elegans
recombinant protein Thermotoga maritima

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Homo sapiens
-
blood
-
Rattus norvegicus
-
brain
-
Homo sapiens
-
brain
-
Rattus norvegicus
-
embryo
-
Arabidopsis thaliana
-
erythrocyte
-
Homo sapiens
-
erythrocyte
-
Rattus norvegicus
-
germ
-
Triticum aestivum
-
leaf
-
Triticum aestivum
-
leaf
-
Zea mays
-
leaf
-
Arabidopsis thaliana
-
leaf
-
Solanum lycopersicum
-
liver
-
Rattus norvegicus
-
oocyte
-
Xenopus laevis
-
seed
-
Triticum aestivum
-
seed
-
Zea mays
-
seed
-
Arabidopsis thaliana
-
seed
-
Solanum lycopersicum
-
seed
-
Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000127
-
VYP-L-isoAsp-GHG as substrate Arabidopsis thaliana
0.00013
-
gamma-globulin as substrate Arabidopsis thaliana
0.000229
-
VYP-L-isoAsp-RR as substrate Arabidopsis thaliana
0.000433
-
ovalbumin as substrate Arabidopsis thaliana
0.000439
-
-
Arabidopsis thaliana
0.001441
-
VYP-L-isoAsp-CA as substrate Arabidopsis thaliana
0.004179
-
KASA-L-isoAsp-LAKY as substrate Arabidopsis thaliana
0.005028
-
VYP-L-isoAsp-HA as substrate Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + gamma-globulin
-
Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + gamma-globulin
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA (isoD) LAKY
-
Escherichia coli S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA (isoD) LAKY
-
Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA (isoD) LAKY
-
Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
Homo sapiens S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
Triticum aestivum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
Zea mays S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
-
Oryza sativa S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Escherichia coli S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Homo sapiens S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Triticum aestivum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Zea mays S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Oryza sativa S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + ovalbumin
-
Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + protein D-aspartate
-
Escherichia coli S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
-
?
S-adenosyl-L-methionine + protein D-aspartate
-
Homo sapiens S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
-
?
S-adenosyl-L-methionine + protein D-aspartate
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Drosophila melanogaster S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Escherichia coli S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Homo sapiens S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Rattus norvegicus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Triticum aestivum S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Zea mays S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Xenopus laevis S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Caenorhabditis elegans S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Solanum lycopersicum S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Oryza sativa S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Thermotoga maritima S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate repair enzyme, methylates abnormal L-isoaspartate residues in proteins which arise spontaneously as a result of aging Arabidopsis thaliana S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-CA
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-GHG
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
Homo sapiens S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
Triticum aestivum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
Zea mays S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
-
Oryza sativa S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-RR
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Escherichia coli
monomer
-
Homo sapiens
monomer
-
Thermotoga maritima
monomer 1 * 28000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
protein repair L-isoaspartyl methyltransferase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Escherichia coli
45
-
recombinant enzyme, expressed in E. coli Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
loses activity rapidly at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 recombinant enzyme, expressed in Escherichia coli Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
Arabidopsis thaliana