Cloned (Comment) | Organism |
---|---|
gene FOMT5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of soluble homodimeric His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Citrus depressa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is an Mg2+-independent O-methyltransferase | Citrus depressa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
recombinant His6-tagged enzyme, gel filtration | Citrus depressa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + quercetin | Citrus depressa | whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product | 4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus depressa | A0A125T1T5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Citrus depressa | - |
pericarp | - |
Citrus depressa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + quercetin | whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product | Citrus depressa | 4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin | - |
? | |
additional information | the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity. Isozyme CdFOMT5 exhibits O-methyltransferase activity for quercetin, naringenin, (-)-epicatechin, and equol using S-adenosyl-L-methionine (SAM) as a methyl donor. The recombinant CdFOMT5 CdFOMT5 can catalyze the O-methylation of at least three hydroxyl groups of quercetin, and di- or tri-O-methylated quercetin products are obtained by this enzymatic reaction. CdFMOT5 prefers flavonol (3-hydroxyflavone) to other flavonoid structures. Thus, substrate structure, especially the C-ring in flavonoids, may strongly affect the substrate preference, including regioselectivity, of CdFOMT5 | Citrus depressa | ? | - |
- |
|
S-adenosyl-L-methionine + quercetin | - |
Citrus depressa | S-adenosyl-L-homocysteine + 3'-methoxyquercetin | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Citrus depressa | S-adenosyl-L-homocysteine + 3-methoxyquercetin | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Citrus depressa | S-adenosyl-L-homocysteine + 5-methoxyquercetin | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Citrus depressa | S-adenosyl-L-homocysteine + 7-methoxyquercetin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42030, recombinant His6-tagged enzyme, sequence calculation, 2 * 42000, recombinant His6-tagged enzyme, SDS-PAGE | Citrus depressa |
Synonyms | Comment | Organism |
---|---|---|
CdFOMT5 | - |
Citrus depressa |
flavonoid-O-methyltransferase | - |
Citrus depressa |
FOMT5 | - |
Citrus depressa |
Mg2+-independent O-methyltransferase | - |
Citrus depressa |
More | see also EC 2.1.1.267 | Citrus depressa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Citrus depressa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Citrus depressa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | recombinant enzyme, over 50% activity within this range | Citrus depressa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Citrus depressa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Citrus depressa | recombinant detagged enzyme, sequence calculation | - |
5.79 |