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Literature summary for 2.1.1.76 extracted from

  • Schmidt, A.; Li, C.; Daniel Jones, A.; Pichersky, E.
    Characterization of a flavonol 3-O-methyltransferase in the trichomes of the wild tomato species Solanum habrochaites (2012), Planta, 236, 839-849.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Solanum habrochaites

Inhibitors

Inhibitors Comment Organism Structure
3'-O-methylmyricetin i.e. laricitrin, inhibitory at substrate concentrations above 0.025 mM Solanum habrochaites

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00055
-
myricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
0.00207
-
3'-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
0.00998
-
7-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
0.01086
-
S-adenosyl-L-methionine in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Solanum habrochaites

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum habrochaites the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl ?
-
?

Organism

Organism UniProt Comment Textmining
Solanum habrochaites
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ column chromatography Solanum habrochaites

Source Tissue

Source Tissue Comment Organism Textmining
trichome glandular trichome Solanum habrochaites
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl Solanum habrochaites ?
-
?
S-adenosyl-L-methionine + 3',5'-O-dimethylmyricetin 18% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3',5'-O-trimethylmyricetin
-
?
S-adenosyl-L-methionine + 3'-O-methylmyricetin 64% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
-
?
S-adenosyl-L-methionine + 3'-O-methylmyricetin i.e. laricitrin, 43% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
-
?
S-adenosyl-L-methionine + 3-O-methylquercetin 3% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + 4'-O-methylkaempferol 11% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,4'-O-dimethylkaempferol
-
?
S-adenosyl-L-methionine + 7-O-methylmyricetin 104% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
-
?
S-adenosyl-L-methionine + kaempferol 53% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3-O-methylkaempferol
-
?
S-adenosyl-L-methionine + myricetin 100% activity Solanum habrochaites S-adenosyl-L-homocysteine + 3-O-methylmyricetin
-
?
S-adenosyl-L-methionine + quercetin 121% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3-O-methylquercetin
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Solanum habrochaites

Synonyms

Synonyms Comment Organism
3-OMT
-
Solanum habrochaites
flavonol 3-O-methyltransferase
-
Solanum habrochaites
MOMT3
-
Solanum habrochaites

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
7-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
0.82
-
myricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
1.66
-
3'-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.94
-
S-adenosyl-L-methionine in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
7-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
290
-
S-adenosyl-L-methionine in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
930
-
3'-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
1490
-
myricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites