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Literature summary for 2.1.1.72 extracted from

  • Ma, B.; Ma, J.; Liu, D.; Guo, L.; Chen, H.; Ding, J.; Liu, W.; Zhang, H.
    Biochemical and structural characterization of a DNA N6-adenine methyltransferase from Helicobacter pylori (2016), Oncotarget, 7, 40965-40977 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, hanging drop vapor diffusion method, using 1.0 M Bis-Tris, pH 9.0, 1.4 M ammonium tartrate. Enzyme in complex with S-adenosyl-L-methionine, hanging drop vapor diffusion method, using 1.0 M Bis-Tris pH 6.0, 14% (w/v) PEG 2000, 0.2 M lithium sulfate Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Helicobacter pylori the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?
S-adenosyl-L-methionine + DNA adenine Helicobacter pylori 26695 the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Helicobacter pylori 26695
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography, Mono S column chromatography, and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?
S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' Helicobacter pylori 26695 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Subunits

Subunits Comment Organism
dimer gel filtration Helicobacter pylori

Synonyms

Synonyms Comment Organism
DNA N6-adenine methyltransferase
-
Helicobacter pylori
M1.HpyAVI
-
Helicobacter pylori
N6-adenine MTase
-
Helicobacter pylori