Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
macronucleus | - |
Tetrahymena thermophila | 31039 | - |
macronucleus | - |
Oxytricha trifallax | 31039 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Tetrahymena thermophila | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Oxytricha trifallax | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Oxytricha trifallax JRB310 | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Tetrahymena thermophila SB210 | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oxytricha trifallax | - |
- |
- |
Oxytricha trifallax JRB310 | - |
- |
- |
Tetrahymena thermophila | - |
- |
- |
Tetrahymena thermophila SB210 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration | Tetrahymena thermophila |
HiTrap Q column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration | Oxytricha trifallax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | Tetrahymena thermophila | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | Oxytricha trifallax | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | Oxytricha trifallax JRB310 | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA | Tetrahymena thermophila SB210 | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA 6mA methyltransferase | - |
Tetrahymena thermophila |
DNA 6mA methyltransferase | - |
Oxytricha trifallax |
MTA1 | catalytic subunit | Tetrahymena thermophila |
MTA1 | catalytic subunit | Oxytricha trifallax |
MTA1c | - |
Tetrahymena thermophila |
MTA1c | - |
Oxytricha trifallax |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme gene disruption mutants fail to complete the sexual cycle when induced to mate and display complete lethality | Tetrahymena thermophila |
malfunction | enzyme gene disruption mutants fail to complete the sexual cycle when induced to mate and display complete lethality | Oxytricha trifallax |