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Literature summary for 2.1.1.72 extracted from

  • Dedkov, V.; Gonchar, D.; Abdurashitov, M.; Udalyeva, S.; Urumceva, L.; Chernukhin, V.; Shiryaeva, E.; Degtyarev, S.
    Cloning and study of new DNA methyltransferase M.AluBI modifying adenine in a recognition site AGCT (2015), Biotecnol. Apl., 32, 3211-3216 .
No PubMed abstract available
Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli RR1 cells Cellulosimicrobium cellulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine in DNA Cellulosimicrobium cellulans
-
 S-adenosyl-L-homocysteine + N6-methyladenine in DNA
-
 ?
S-adenosyl-L-methionine + adenine in DNA Cellulosimicrobium cellulans B
-
 S-adenosyl-L-homocysteine + N6-methyladenine in DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium cellulans
-
-
-
Cellulosimicrobium cellulans B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
P-11 phosphocellulose column chromatography, heparin-Sepharose column chromatography, Sephacryl S-200 gel filtration, and hydroxyapatite column chromatography Cellulosimicrobium cellulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine in DNA
-
 Cellulosimicrobium cellulans S-adenosyl-L-homocysteine + N6-methyladenine in DNA
-
 ?
S-adenosyl-L-methionine + adenine in DNA
-
 Cellulosimicrobium cellulans B S-adenosyl-L-homocysteine + N6-methyladenine in DNA
-
 ?
S-adenosyl-L-methionine + lambda phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans ?
-
 ?
S-adenosyl-L-methionine + lambda phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans B ?
-
 ?
S-adenosyl-L-methionine + T7 phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans ?
-
 ?
S-adenosyl-L-methionine + T7 phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans B ?
-
 ?

Synonyms

Synonyms Comment Organism
adenine-(N6)-DNA methyltransferase
-
 Cellulosimicrobium cellulans
M.AluBI
-
 Cellulosimicrobium cellulans