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Literature summary for 2.1.1.72 extracted from

  • Elsawy, H.; Chahar, S.
    Increasing DNA substrate specificity of the EcoDam DNA-(adenine N6)-methyltransferase by site-directed mutagenesis (2014), Biochemistry, 79, 1262-1266.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HMS174 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
L122A the mutant is almost inactive on unmethylated DNA. The variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA with 35-40% of wild type activity Escherichia coli
L122A/P134A the variant methylates hemimethylated DNA with 80% of wild type activity Escherichia coli
L122A/V133L the mutant shows increased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli
L122A/V133L the variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA Escherichia coli
L122G the mutant has no preference for hemimethylated substrate and is about 1.5fold more active than the wild type enzyme on the hemimethylated DNA substrate Escherichia coli
L122I the variant methylates hemimethylated DNA with 98% of wild type activity Escherichia coli
L122S the variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA with 35-40% of wild type activity Escherichia coli
L122T the variant methylates hemimethylated DNA with 90% of wild type activity Escherichia coli
L122V the variant methylates hemimethylated DNA with 95% of wild type activity Escherichia coli
P134A the variant methylates hemimethylated DNA with 100% of wild type activity Escherichia coli
V133A the mutant shows increased activity with hemimethylated DNA compared to the wild type enzyme (160%) Escherichia coli
V133I the mutant shows decreased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli
V133L the mutant shows most strongly decreased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli
V133S the mutant shows strongly decreased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the wild type enzyme is active on hemimethylated and unmethylated DNA substrates ?
-
?
S-adenosyl-L-methionine + DNA adenine Escherichia coli
-
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEE8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the wild type enzyme is active on hemimethylated and unmethylated DNA substrates Escherichia coli ?
-
?
S-adenosyl-L-methionine + DNA adenine
-
Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
Dam
-
Escherichia coli
DNA-(adenine N6)-methyltransferase
-
Escherichia coli