Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HMS174 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L122A | the mutant is almost inactive on unmethylated DNA. The variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA with 35-40% of wild type activity | Escherichia coli |
L122A/P134A | the variant methylates hemimethylated DNA with 80% of wild type activity | Escherichia coli |
L122A/V133L | the mutant shows increased activity with hemimethylated DNA compared to the wild type enzyme | Escherichia coli |
L122A/V133L | the variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA | Escherichia coli |
L122G | the mutant has no preference for hemimethylated substrate and is about 1.5fold more active than the wild type enzyme on the hemimethylated DNA substrate | Escherichia coli |
L122I | the variant methylates hemimethylated DNA with 98% of wild type activity | Escherichia coli |
L122S | the variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA with 35-40% of wild type activity | Escherichia coli |
L122T | the variant methylates hemimethylated DNA with 90% of wild type activity | Escherichia coli |
L122V | the variant methylates hemimethylated DNA with 95% of wild type activity | Escherichia coli |
P134A | the variant methylates hemimethylated DNA with 100% of wild type activity | Escherichia coli |
V133A | the mutant shows increased activity with hemimethylated DNA compared to the wild type enzyme (160%) | Escherichia coli |
V133I | the mutant shows decreased activity with hemimethylated DNA compared to the wild type enzyme | Escherichia coli |
V133L | the mutant shows most strongly decreased activity with hemimethylated DNA compared to the wild type enzyme | Escherichia coli |
V133S | the mutant shows strongly decreased activity with hemimethylated DNA compared to the wild type enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the wild type enzyme is active on hemimethylated and unmethylated DNA substrates | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEE8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the wild type enzyme is active on hemimethylated and unmethylated DNA substrates | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | - |
Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Dam | - |
Escherichia coli |
DNA-(adenine N6)-methyltransferase | - |
Escherichia coli |