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Literature summary for 2.1.1.72 extracted from
- The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM methylates DNA in a distributive manner (2012), Nucleic Acids Res., 40, 1708-1716.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Caulobacter vibrioides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Caulobacter vibrioides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caulobacter vibrioides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography, gel filtration | Caulobacter vibrioides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme has a high specificity for GANTC sites, with only minor preferences at the central position. It slightly (1.5fold) prefers hemimethylated DNA over methylated DNA | Caulobacter vibrioides | ? | - |
? |  |
| S-adenosyl-L-methionine + DNA adenine | 129-mer DNA or pUC19 DNA are used as substrates | Caulobacter vibrioides | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Caulobacter vibrioides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CcrM | - |
Caulobacter vibrioides |
| cell-cycle regulating MTase | - |
Caulobacter vibrioides |
| DNA-(adenine-N6)-methyltransferase | - |
Caulobacter vibrioides |
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Release 2023.1 (January 2023)
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