Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli RRI cells | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | enzyme activity decreases 100fold in the presence of 100 mM K+ | Geobacillus stearothermophilus | |
Mg2+ | enzyme activity decreases in the presence of Mg2+ concentrations above 2.5 mM | Geobacillus stearothermophilus | |
Na+ | enzyme activity decreases 100fold in the presence of 100 mM Na+ | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000098 | - |
T7 DNA adenine | in 33 mM Tris-acetate, pH 7.9, 10 mM magnesium acetate, 66 mM potassium acetate, 1 mM dithiothreitol, at 55°C | Geobacillus stearothermophilus | |
0.0058 | - |
S-adenosyl-L-methionine | in 33 mM Tris-acetate, pH 7.9, 10 mM magnesium acetate, 66 mM potassium acetate, 1 mM dithiothreitol, at 55°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Geobacillus stearothermophilus | the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Geobacillus stearothermophilus SE-589 | the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + T7 DNA adenine | Geobacillus stearothermophilus | - |
S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + T7 DNA adenine | Geobacillus stearothermophilus SE-589 | - |
S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus SE-589 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phosphocellulose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates | Geobacillus stearothermophilus | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates | Geobacillus stearothermophilus SE-589 | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + T7 DNA adenine | - |
Geobacillus stearothermophilus | S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + T7 DNA adenine | - |
Geobacillus stearothermophilus SE-589 | S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA methyltransferase | - |
Geobacillus stearothermophilus |
M2.BstSEI | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
T7 DNA adenine | in 33 mM Tris-acetate, pH 7.9, 10 mM magnesium acetate, 66 mM potassium acetate, 1 mM dithiothreitol, at 55°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Geobacillus stearothermophilus |