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Literature summary for 2.1.1.72 extracted from

  • Elsawy, H.; Podobinschi, S.; Chahar, S.; Jeltsch, A.
    Transition from EcoDam to T4Dam DNA recognition mechanism without loss of activity and specificity (2009), ChemBioChem, 10, 2488-2493.
    View publication on PubMed
Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
K9A the mutant shows low activity and loss of recognition of Gua1 Escherichia coli
K9A the mutant shows strongly reduced methylation activity towards the sequence GATC Escherichia coli
K9A/Y138R the double mutant is highly active and specific Escherichia coli
Y138R the mutant which carries both base Gua1 recognition elements (K9 from EcoDam) is fully active and specific, about 2fold more active than the wild type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Escherichia coli Dam methylates the adenine residue in GATC sites S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Dam methylates the adenine residue in GATC sites Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Synonyms

Synonyms Comment Organism
Dam
-
 Escherichia coli
DNA-(adenine N6)-methyltransferase
-
 Escherichia coli