Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K9A | the mutant shows low activity and loss of recognition of Gua1 | Escherichia coli |
K9A | the mutant shows strongly reduced methylation activity towards the sequence GATC | Escherichia coli |
K9A/Y138R | the double mutant is highly active and specific | Escherichia coli |
Y138R | the mutant which carries both base Gua1 recognition elements (K9 from EcoDam) is fully active and specific, about 2fold more active than the wild type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | Dam methylates the adenine residue in GATC sites | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Dam methylates the adenine residue in GATC sites | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dam | - |
Escherichia coli |
DNA-(adenine N6)-methyltransferase | - |
Escherichia coli |