Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL2-Blue cells | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phosphocellulose column chromatography and Blue Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | Dam dimerizes on short, synthetic DNA, resulting in enhanced catalysis, however, dimerization is not observed on large genomic DNA | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Dam | - |
Escherichia coli |
DNA adenine methyltransferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
DNA adenine | in 100 mM Tris (pH 8.0), 1 mM EDTA, 1 mM dithiothreitol, and 0.2 mg/ml bovine serum albumin, at 22°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | DNA adenine methyltransferase is essential for proper DNA replication timing, gene regulation, and mismatch repair | Escherichia coli |