Literature summary for 2.1.1.72 extracted from

Mashhoon, N.; Carroll, M.; Pruss, C.; Eberhard, J.; Ishikawa, S.; Estabrook, R.A.; Reich, N.
Functional characterization of Escherichia coli DNA adenine methyltransferase, a novel target for antibiotics (2004), J. Biol. Chem., 279, 52075-52081.

Search for more literature for 2.1.1.72

Application

Application	Comment	Organism
pharmacology	enzyme is a target for antibiotics and antbiotic development	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression as His-tagged protein in strain XL-2Blue	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
methylated DNA	mixed-type inhibition against S-adenosyl-L-methionine, uncompetitive against unmethylated DNA	Escherichia coli
S-adenosyl-L-homocysteine	competitive against S-adenosyl-L-methionine, uncompetitive against DNA	Escherichia coli
sinefungin	enzyme-DNA-inhibitor complex formation and structure	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics, kinetic mechanism, thermodynamics	Escherichia coli
0.000017	-	adenine in DNA	pH 8.0, 37°C	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + DNA adenine	Escherichia coli	enzyme is a critical regulator of bacterial virulence	S-adenosyl-L-homocysteine + DNA 6-methylaminopurine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from strain XL-2Blue	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA	ordered bi bi mechanism with S-adenosyl-L-methionine binding first, fast methyl transfer, slow product release	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + adenine in DNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N6-methyladenine in DNA	-	?
S-adenosyl-L-methionine + DNA adenine	enzyme is a critical regulator of bacterial virulence	Escherichia coli	S-adenosyl-L-homocysteine + DNA 6-methylaminopurine	-	?
S-adenosyl-L-methionine + DNA adenine	DNA substrate from calf thymus, methylation of adjacent GATC sites is distributive with DNA derived from a genetic element that controls the transcription of the adjacent genes, the first methylation event is followed by enzyme release	Escherichia coli	S-adenosyl-L-homocysteine + DNA 6-methylaminopurine	-	?

Synonyms

Synonyms	Comment	Organism
DNA adenine methyltransferase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0155	-	adenine in DNA	pH 8.0, 37°C	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)